Colloidal cold filtrates as metal substrates for surface-enhanced infrared absorption spectroscopy

被引：55

作者：

Seelenbinder, JA ^{[1
]}

Brown, CW

Pivarnik, P

Rand, AG

机构：

[1] Univ Rhode Isl, Partnership Sensors & Surface Technol, Dept Chem, Kingston, RI 02881 USA

[2] Univ Rhode Isl, Partnership Sensors & Surface Technol, Dept Food Sci & Nutr, Kingston, RI 02881 USA

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 10期

关键词：

D O I：

10.1021/ac981170l

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method for obtaining surface-enhanced infrared absorption (SEIRA) spectra of antibodies and antibody/antigen complexes has been developed. Antibodies attached to colloidal gold particles and then collected by filtration onto porous polyethylene membranes show enhanced spectral bands at 1080 and 990 cm(-1) regardless of the antibody specificity. Attachment of a model antigen, glucose oxidase, to its specific antibody/colloid complex prior to collection produces enhanced bands at 1540, 1395, and 1250 cm(-1). Similarly, when the antigen Salmonella is attached to its specific antibody/colloid complex prior to collection, a new enhanced band is observed at 1015 cm(-1). Similarities and differences of the SEIRA spectra obtained on gold colloid are compared to previous work on gold films.

引用

页码：1963 / 1966

页数：4

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