Inhibitory Effects and Molecular Mechanisms of Selenium-Containing Tea Polysaccharides on Human Breast Cancer MCF-7 Cells

被引：112

作者：

He, Nianwu ^{[1
,2
]}

Shi, Xiaolong ^{[1
]}

Zhao, Yan ^{[3
]}

Tian, Lingmin ^{[1
]}

Wang, Dongying ^{[1
]}

Yang, Xingbin ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Coll Food Engn & Nutr Sci, Key Lab, Minist Educ Med Resource & Nat Pharmaceut Chem, Xian 710062, Peoples R China

[2] Shangluo Univ, Dept Biopharmaceut Engn, Shangluo 726000, Peoples R China

[3] Fourth Mil Med Univ, Sch Pharm, Xian 710032, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2013年 / 61卷 / 03期

基金：

中国国家自然科学基金;

关键词：

tea polysaccharide; selenium; breast cancer; MCF-7; cells; apoptosis; GREEN TEA; CYCLE ARREST; ANTIOXIDANT ACTIVITIES; MITOCHONDRIA PATHWAY; ANTITUMOR ACTIVITIES; CAMELLIA-SINENSIS; HUMAN COLON; IN-VITRO; APOPTOSIS; PROTEINS;

D O I：

10.1021/jf3036929

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Dietary supplementation of selenium-enriched tea is known to have an anticancer health benefit. This study was to investigate the inhibitory effect of selenium-containing tea polysaccharides (Se-GTPs) from a new variety of selenium-enriched Ziyang green tea against human MCF-7 breast cancer cells. Se-GTPs dose-dependently exhibited an effective cell growth inhibition with an IC50 of 140.1 mu g/mL by inducing MCF-7 cancer cells to undergo G2/M phase arrest and apoptosis. The blockade of cell cycle was associated with an up-regulation of p53 expression, but not CDK2. Se-GTPs clearly triggered the mitochondrial apoptotic pathway, as indicated by an increase in Bax/Bcl-2 ratio and subsequent caspase-3 and caspase-9 activation. It was also found that the generation of intracellular ROS is a critical mediator in Se-GTPs-induced cell growth inhibition. These findings suggest that Se-GTPs may serve as a potential novel dietary agent for human breast cancer chemoprevention.

引用

页码：579 / 588

页数：10

共 45 条

[1]

A Dietary Anthocyanidin Delphinidin Induces Apoptosis of Human Prostate Cancer PC3 Cells In vitro and In vivo: Involvement of Nuclear Factor-κB Signaling [J].