CXCL10 and IL-6 induce chemotaxis in human trophoblast cell lines

被引：64

作者：

Dominguez, F. ^{[1
]}

Martinez, S. ^{[1
]}

Quinonero, A. ^{[1
]}

Loro, F. ^{[1
]}

Horcajadas, J. A. ^{[1
]}

Pellicer, A. ^{[1
,2
]}

Simon, C. ^{[1
,2
]}

机构：

[1] Univ Valencia, FIVI, Valencia 46015, Spain

[2] Univ Valencia, Sch Med, Dept Pediat Obstet & Gynecol, Valencia 46010, Spain

来源：

MOLECULAR HUMAN REPRODUCTION | 2008年 / 14卷 / 07期

关键词：

CXCL10; chemotaxis; implantation; IL-6;

D O I：

10.1093/molehr/gan032

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The investigation of trophoblast chemoattractive molecules in humans is of high interest for the reproductive field. Current evidence in ruminants demonstrates that CXCL10, formerly the interferon-gamma-inducible protein 10 (IP-10), is a potent chemotactic molecule implicated in the migration of trophoblast cells during early gestation. The aim of this work was to explore the existence of CXCL10/CXCR3 in the human model. Furthermore, chemotaxis assays were performed to demonstrate CXCL10 chemotactic activity in the human trophoblast cell lines JEG-3 and AC-1M88. Surprisingly, the conditioned media from epithelial endometrial cells (EEC) induced the highest trophoblast migration rate. Cytokine and chemokine membrane protein arrays were used to identify the secreted protein profile of EEC-conditioned media, and IL-6 was found to be the most abundant and CXCL13 the second most abundant molecule. Using a chemotaxis assay on AC-IM88, IL-6 antibody blocked the effect of EEC, indicating IL-6 to be an effective chemoattractive factor for trophoblast cells in the human model.

引用

页码：423 / 430

页数：8

共 31 条

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