Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin

被引：553

作者：

Niwa, R

Nagata-Ohashi, K

Takeichi, M

Mizuno, K

Uemura, T ^{[1
]}

机构：

[1] Kyoto Univ, Inst Virus Res, Dept Mol Genet, Kyoto 6068507, Japan

[2] Kyoto Univ, Grad Sch Sci, Sakyo Ku, Kyoto 6068502, Japan

[3] Kyoto Univ, Grad Sch Biostudies, Sakyo Ku, Kyoto 6068502, Japan

[4] Tohoku Univ, Grad Sch Life Sci, Dept Biomol Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

[5] Japan Sci & Technol Corp, CREST, Tsukuba, Ibaraki, Japan

来源：

CELL | 2002年 / 108卷 / 02期

关键词：

D O I：

10.1016/S0092-8674(01)00638-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ADF (actin-depolymerizing factor)/cofilin family is a stimulus-responsive mediator of actin dynamics. In contrast to the mechanisms of inactivation of ADF/cofilin by kinases such as LIM-kinase 1 (LIMK1), much less is known about its reactivation through dephosphorylation. Here we report Slingshot (SSH), a family of phosphatases that have the property of F actin binding. In Drosophila, loss of ssh function dramatically increased levels of both F actin and phospho-cofilin (P cofilin) and disorganized epidermal cell morphogenesis. In mammalian cells, human SSH homologs (hSSHs) suppressed LIMK1-induced actin reorganization. Furthermore, SSH and the hSSHs dephosphorylated P cofilin in cultured cells and in cell-free assays. Our results strongly suggest that the SSH family plays a pivotal role in actin dynamics by reactivating ADF/cofilin in vivo.

引用

页码：233 / 246

页数：14

共 73 条

[1] A COFILIN-LIKE PROTEIN IS INVOLVED IN THE REGULATION OF ACTIN ASSEMBLY IN DEVELOPING SKELETAL-MUSCLE [J].

ABE, H ;

OHSHIMA, S ;

OBINATA, T .

JOURNAL OF BIOCHEMISTRY, 1989, 106 (04) :696-702

[2] Xenopus laevis actin-depolymerizing factor cofilin: A phosphorylation-regulated protein essential for development [J].

Abe, H ;

Obinata, T ;

Minamide, LS ;

Bamburg, JR .

JOURNAL OF CELL BIOLOGY, 1996, 132 (05) :871-885

[3] The genome sequence of Drosophila melanogaster [J].

Adams, MD ;

Celniker, SE ;

Holt, RA ;

Evans, CA ;

Gocayne, JD ;

Amanatides, PG ;

Scherer, SE ;

Li, PW ;

Hoskins, RA ;

Galle, RF ;

George, RA ;

Lewis, SE ;

Richards, S ;

Ashburner, M ;

Henderson, SN ;

Sutton, GG ;

Wortman, JR ;

Yandell, MD ;

Zhang, Q ;

Chen, LX ;

Brandon, RC ;

Rogers, YHC ;

Blazej, RG ;

Champe, M ;

Pfeiffer, BD ;

Wan, KH ;

Doyle, C ;

Baxter, EG ;

Helt, G ;

Nelson, CR ;

Miklos, GLG ;

Abril, JF ;

Agbayani, A ;

An, HJ ;

Andrews-Pfannkoch, C ;

Baldwin, D ;

Ballew, RM ;

Basu, A ;

Baxendale, J ;

Bayraktaroglu, L ;

Beasley, EM ;

Beeson, KY ;

Benos, PV ;

Berman, BP ;

Bhandari, D ;

Bolshakov, S ;

Borkova, D ;

Botchan, MR ;

Bouck, J ;

Brokstein, P .

SCIENCE, 2000, 287 (5461) :2185-2195

[4] REACTIVATION OF PHOSPHORYLATED ACTIN DEPOLYMERIZING FACTOR AND IDENTIFICATION OF THE REGULATORY SITE [J].

AGNEW, BJ ;

MINAMIDE, LS ;

BAMBURG, JR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (29) :17582-17587

[5] Phosphorylation of cofilin by LIM-kinase is necessary for semaphorin 3A-induced growth cone collapse [J].

Aizawa, H ;

Wakatsuki, S ;

Ishii, A ;

Moriyama, K ;

Sasaki, Y ;

Ohashi, K ;

Sekine-Aizawa, Y ;

Sehara-Fujisawa, A ;

Mizuno, K ;

Goshima, Y ;

Yahara, I .

NATURE NEUROSCIENCE, 2001, 4 (04) :367-373

[6] LIM-kinase 2 induces formation of stress fibres, focal adhesions and membrane blebs, dependent on its activation by Rho-associated kinase-catalysed phosphorylation at threonine-505 [J].

Amano, T ;

Tanabe, K ;

Eto, T ;

Narumiya, S ;

Mizuno, K .

BIOCHEMICAL JOURNAL, 2001, 354 :149-159

[7]

Ambach A, 2000, EUR J IMMUNOL, V30, P3422, DOI 10.1002/1521-4141(2000012)30:12<3422::AID-IMMU3422>3.0.CO

[8]

2-J

[9] Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase [J].

Arber, S ;

Barbayannis, FA ;

Hanser, H ;

Schneider, C ;

Stanyon, CA ;

Bernard, O ;

Caroni, P .

NATURE, 1998, 393 (6687) :805-809

[10] ONE OF THE PROTEIN PHOSPHATASE-1 ISOENZYMES IN DROSOPHILA IS ESSENTIAL FOR MITOSIS [J].

AXTON, JM ;

DOMBRADI, V ;

COHEN, PTW ;

GLOVER, DM .

CELL, 1990, 63 (01) :33-46

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