The zinc-finger proto-oncogene Gfi-1b is essential for development of the erythroid and megakaryocytic lineages

被引:186
作者
Saleque, S
Cameron, S
Orkin, SH [1 ]
机构
[1] Harvard Univ, Childrens Hosp, Sch Med, Dana Farber Canc Inst,Dept Pediat Oncol, Boston, MA 02115 USA
[2] Howard Hughes Med Inst, Boston, MA 02115 USA
[3] MIT, Dept Biol, Cambridge, MA 02139 USA
[4] Howard Hughes Med Inst, Cambridge, MA 02139 USA
关键词
transcription factor; hematopoiesis; development; oncogene;
D O I
10.1101/gad.959102
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Gfi-1 and Gfi-1b are novel proto-oncogenes identified by retroviral insertional mutagenesis. By gene targeting, we establish that Gfi-1b is required for the development of two related blood lineages, erythroid and megakaryocytic, in mice. Gfi-1b(-/-) embryonic stem cells fail to contribute to red cells of adult chimeras. Gfi-1b(-/-) embryos exhibit delayed maturation of primitive erythrocytes and subsequently die with failure to produce definitive enucleated erythrocytes. The fetal liver of mutant mice contains erythroid and megakaryocytic precursors arrested in their development. Myelopoiesis is normal. Therefore, Gfi-1b is an essential transcriptional regulator of erythroid and megakaryocyte development.
引用
收藏
页码:301 / 306
页数:6
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