CTX, a novel molecule specifically expressed on the surface of cortical thymocytes in Xenopus

被引:58
作者
Chretien, I
Robert, J
Marcuz, A
GarciaSanz, JA
Courtet, M
DuPasquier, L
机构
[1] Basel Institute for Immunology, Basel
[2] Dept. Microbiol. Immunol. Med. Ctr., University of Rochester, Rochester
[3] Basel Institute for Immunology, CH-4005 Basel
关键词
Xenopus; evolution; immunoglobulin domain; multigene family; selection;
D O I
10.1002/eji.1830260409
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
CTX, a novel developmentally regulated type-I transmembrane protein is expressed specifically by a large fraction of cortical thymocytes in the amphibian Xenopus. This apparently monomeric 55-kDa glycoprotein is composed of two immunoglobulin domains, one variable (V) and one constant (C2 type), followed by a transmembrane and a 64-amino acid cytoplasmic domain. The first immunoglobulin domain is a V-J segment that is generated without gene rearrangement. In the genome, the V and C2 domains are both encoded by two half-domain exons. Two CTX loci are found per haploid genome, and they exhibit sequence differences with a high replacing/silent ratio in the CDR1-like region of the V domain. suggesting that these differences were selected. The cytoplasmic domain contains a motif that is highly conserved evolutionarily in several types of proteins, including adenylyl cyclases. Based on its unique tissue distribution. the variability of its V region and the motif of its cytoplasmic domain. CTX is a candidate for a new type of specific signaling molecule involved in thymocyte selection.
引用
收藏
页码:780 / 791
页数:12
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