Kinetic analysis of ethanol production by an acetate-resistant strain of recombinant Zymomonas mobilis

被引：29

作者：

Jeon, YJ ^{[1
]}

Svenson, CJ ^{[1
]}

Joachimsthal, EL ^{[1
]}

Rogers, PL ^{[1
]}

机构：

[1] Univ New S Wales, Dept Biotechnol, Sydney, NSW 2052, Australia

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 10期

关键词：

acetate-tolerant mutant recombinant Zymomonas mobilis; glucose/xylose fermentation; lignocellulosic hydrolysates;

D O I：

10.1023/A:1015546521000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Zymomonas mobilis ZM4/Ac-R (pZB5), a mutant recombinant strain with increased acetate resistance, has been isolated following electroporation of Z. mobilis ZM4/Ac-R. This mutant strain showed enhanced kinetic characteristics in the presence of 12 g sodium acetate l(-1) at pH 5 in batch culture on 40 g glucose, 40 g xylose l(-1) medium when compared to ZM4 (pZB5). In continuous culture, there was evidence of increased maintenance energy requirements/uncoupling of metabolism for ZM4/Ac-R (pZB5) in the presence of sodium acetate; a result confirmed by analysis of the effect of acetate on other strains of Z. mobilis.

引用

页码：819 / 824

页数：6

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