Actinobacillus pleuropneumoniae does not require urease activity to produce acute swine pleuropneumonia

被引：8

作者：

Cabrero, RIT ^{[1
]}

VazquezBoland, JA ^{[1
]}

Gutierrez, CB ^{[1
]}

RodriguezBarbosa, JI ^{[1
]}

RodriguezFerri, EF ^{[1
]}

机构：

[1] UNIV COMPLUTENSE,FAC VET,DEPT PATOL ANIM 1,UNIDAD MICROBIOL & INMUNOL,E-28040 MADRID,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 148卷 / 01期

关键词：

Actinobacillus pleuropneumoniae; urease; transposon mutagenesis; virulence; swine pleuropneumonia;

D O I：

10.1016/S0378-1097(97)00012-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The role in virulence of Actinobacillus pleuropneumoniae urease activity was investigated. A urease-negative mutant was isolated following transposon mutagenesis with a mini-Tn10 derivative. Both the parent strain and the urease-negative mutant exhibited identical LD(50) values in a murine infection model. Pig challenge confirmed that the urease-negative mutant was fully virulent, since experimental inoculation with 5x10(7) colony forming units resulted in an acute disease indistinguishable from that produced by the wild-type strain at the same dose. Our results demonstrate that urease activity is not required for the development of acute pleuropneumonia.

引用

页码：53 / 57

页数：5

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