A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transiently transfected cells

被引：38

作者：

Lamm, GM ^{[1
]}

Steinlein, P ^{[1
]}

Cotten, M ^{[1
]}

Christofori, G ^{[1
]}

机构：

[1] RES INST MOL PATHOL IMP,A-1030 VIENNA,AUSTRIA

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 23期

关键词：

D O I：

10.1093/nar/25.23.4855

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell types, A plasmid carrying green fluorescent protein (GFP) is co-transfected with an expression vector encoding the gene of interest, Subsequently cells are stained with propidium iodide and, utilising flow cytometry, transfected, GFP-expressing single cells are detected and apoptotic cells in this population are identified by their DNA content of <2 N, The method detects apoptosis as reliably as established methods using in situ nick-end labelling but is faster, easier and less expensive.

引用

页码：4855 / 4857

页数：3

共 24 条

[1] Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery [J].