The CD94 and NKG2-A C-type lectins covalently assemble to form a natural killer cell inhibitory receptor for HLA class I molecules

被引:256
作者
Carretero, M
Cantoni, C
Bellon, T
Bottino, C
Biassoni, R
Rodriguez, A
PerezVillar, JJ
Moretta, L
Moretta, A
LopezBotet, M
机构
[1] UNIV AUTONOMA MADRID,HOSP PRINCESA,SERV IMMUNOL,E-28006 MADRID,SPAIN
[2] CTR BIOTECNOL AVANZATE,GENOA,ITALY
[3] IST SCI STUDIO & CURA TUMORI,GENOA,ITALY
[4] UNIV BRESCIA,DIPARTIMENTO SCI BIOMED & BIOTECNOL,BRESCIA,ITALY
[5] UNIV GENOA,IST ISTOL,GENOA,ITALY
[6] UNIV GENOA,IST PATOL GEN,GENOA,ITALY
关键词
natural killer cell; cytotoxicity; major histocompatibility complex;
D O I
10.1002/eji.1830270230
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
CD94, a type II membrane protein containing a C-type lectin domain, has been shown to be involved in natural killer (NK) cell-mediated recognition of different HLA allotypes. The inhibitory form of the CD94 receptor has recently been identified by the specific monoclonal antibody (mAb) Z199. Herein, we demonstrate that the inhibitory receptor is in fact a complex formed by the covalent association of CD94 with the NKG2-A molecule (Mr similar to 43 kDa), another member of the C-type lectin superfamily, and that Z199 mAb specifically recognize NKG2-A molecules. Although the NKG2-A-encoding cDNA has been known for several years, the corresponding protein and its possible function remained undefined. Moreover, we show that the NKG2-B protein, an alternatively spliced product of the NKG2-A gene, can also assemble with CD94. Remarkably, both NKG2-A and NKG2-B proteins contain cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIM). This may provide the molecular basis of the inhibitory function mediated by the CD94/NKG2-A receptor complexes.
引用
收藏
页码:563 / 567
页数:5
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