Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis

被引：7

作者：

Hernandez-Cruz, E. ^{[1
]}

Gonzalez-Cabriales, J. J. ^{[1
]}

Ordaz-Pichardo, C. ^{[1
]}

de la Cruz-Hernandez, N. I. ^{[2
]}

Flores-Gutierrez, G. H. ^{[1
]}

机构：

[1] Univ Autonoma Tamaulipas, Unidad Acad Multidisciplinaria Reynosa Aztlan, Reynosa, Tamaulipas, Mexico

[2] Univ Autonoma Tamaulipas, Fac Med Vet & Zootecnia Dr Norberto Trevino Zapat, Victoria, Tamaulipas, Mexico

来源：

JOURNAL OF HELMINTHOLOGY | 2009年 / 83卷 / 04期

关键词：

CEREBROSPINAL-FLUID; TAENIA-SOLIUM; MONOCLONAL-ANTIBODIES; MYCOPLASMA-BOVIS; NEUROCYSTICERCOSIS; ELISA; CELLULOSAE; DIAGNOSIS; ANTIGEN; MEXICO;

D O I：

10.1017/S0022149X09270866

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P < 0.05, kappa = 0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.

引用

页码：333 / 337

页数：5

共 29 条

[1]

Agudelo Piedad, 2005, Biomed., V25, P518

[2]

ARAMBULO PV, 1978, ACTA TROP, V35, P63

[3]

BAVEJA U K, 1990, Journal of Communicable Diseases, V22, P55

[4]

Biswas Rakhi, 2004, Rev. Inst. Med. trop. S. Paulo, V46, P249, DOI 10.1590/S0036-46652004000500003

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6]

BRUCKNER DA, 1985, PEDIATR CLIN N AM, V32, P1063

[7] The seroprevalence of Taenia solium cysticercosis among epileptic patients in Leon, Nicaragua, as evaluated by ELISA and western blotting [J].

Bucardo, F ;

Meza-Lucas, A ;

Espinoza, F ;

García-Jerónimo, RC ;

García-Rodea, R ;

Correa, D .

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 2005, 99 (01) :41-45

[8] HUMAN NEUROCYSTICERCOSIS - COMPARISON OF ENZYME-IMMUNOASSAY CAPTURE TECHNIQUES BASED ON MONOCLONAL AND POLYCLONAL ANTIBODIES FOR THE DETECTION OF PARASITE PRODUCTS IN CEREBROSPINAL-FLUID [J].

CORREA, D ;

SANDOVAL, MA ;

HARRISON, LJS ;

PARKHOUSE, RME ;

PLANCARTE, A ;

MEZALUCAS, A ;

FLISSER, A .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1989, 83 (06) :814-816

[9]

COSTA J M, 1986, Arquivos de Neuro-Psiquiatria, V44, P15

[10]

Ferreira Andreia P., 1997, Revista do Instituto de Medicina Tropical de Sao Paulo, V39, P29, DOI 10.1590/S0036-46651997000100006

← 1 2 3 →