The NSm proteins of Rift Valley fever virus are dispensable for maturation, replication and infection

被引：103

作者：

Gerrard, Sonja R.

Bird, Brian H.

Albarino, Cesar G.

Nichol, Stuart T.

机构：

[1] Univ Michigan, Sch Publ Hlth, Dept Epidemiol, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Sch Med, Dept Immunol & Microbiol, Ann Arbor, MI 48109 USA

[3] Ctr Dis Control & Prevent, Div Viral & Rickettsial Dis, Special Pathogens Branch, Atlanta, GA 30333 USA

[4] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA

来源：

VIROLOGY | 2007年 / 359卷 / 02期

关键词：

plasmid-based genetics; Rift Valley fever virus; virus replication; non-structural proteins; accessory proteins; phlebovirus; bunyavirus; vector-borne;

D O I：

10.1016/j.virol.2006.09.035

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rift Valley fever (RVF) virus belongs to the Bunyaviridae family of segmented negative-strand RNA viruses and causes mosquito-borne disease in sub-Saharan Africa. We report the development of a T7 RNA polymerase-driven plasmid-based genetic system for the virulent Egyptian isolate, ZH501. We have used this system to rescue a virus that has a 387 nucleotide deletion on the genomic M segment that eliminates the coding region for two non-structural proteins known as NSm. This virus, Delta NSm rZH501, is indistinguishable from the parental ZH501 strain with respect to expression of structural proteins and growth in cultured mammalian cells. Published by Elsevier Inc.

引用

收藏

页码：459 / 465

页数：7

相关论文

共 24 条

[1] Efficient cDNA-based rescue of La Crosse bunyaviruses expressing or lacking the nonstructural protein NSs [J].

Blakqori, G ;

Weber, F .

JOURNAL OF VIROLOGY, 2005, 79 (16) :10420-10428

[2] PRODUCTION AND CHARACTERIZATION OF ARBOVIRUS ANTIBODY IN MOUSE ASCITIC FLUID [J].

BRANDT, WE ;

BUESCHER, EL ;

HETRICK, FM .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1967, 16 (03) :339-&

[3] Rescue of a segmented negative-strand RNA virus entirely from cloned complementary DNAs [J].

Bridgen, A ;

Elliott, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (26) :15400-15404

[4] Bunyamwera bunyavirus nonstructural protein NSs is a nonessential gene product that contributes to viral pathogenesis [J].

Bridgen, A ;

Weber, F ;

Fazakerley, JK ;

Elliott, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (02) :664-669

[5] Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter [J].

Buchholz, UJ ;

Finke, S ;

Conzelmann, KK .

JOURNAL OF VIROLOGY, 1999, 73 (01) :251-259

[6] MESSENGER-RNA OF THE M-SEGMENT RNA OF RIFT-VALLEY FEVER VIRUS [J].

COLLETT, MS .

VIROLOGY, 1986, 151 (01) :151-156

[7] Synthesis, proteolytic processing and complex formation of N-terminally nested precursor proteins of the Rift Valley fever virus glycoproteins [J].

Gerrard, Sonja R. ;

Nichol, Stuart T. .

VIROLOGY, 2007, 357 (02) :124-133

[8] Characterization of the Golgi retention motif of Rift Valley fever virus GN glycoprotein [J].

Gerrard, SR ;

Nichol, ST .

JOURNAL OF VIROLOGY, 2002, 76 (23) :12200-12210

[9] Rescue of infectious Rift Valley fever virus entirely from cDNA, analysis of virus lacking the NSs gene, and expression of a foreign gene [J].

Ikegami, T ;

Won, S ;

Peters, CJ ;

Makino, S .

JOURNAL OF VIROLOGY, 2006, 80 (06) :2933-2940

[10] RIFT-VALLEY FEVER VIRUS M SEGMENT - USE OF RECOMBINANT VACCINIA VIRUSES TO STUDY PHLEBOVIRUS GENE-EXPRESSION [J].

KAKACH, LT ;

WASMOEN, TL ;

COLLETT, MS .

JOURNAL OF VIROLOGY, 1988, 62 (03) :826-833