Sensitive determination of docetaxel in human plasma by liquid-liquid extraction and reversed-phase high-performance liquid chromatography

被引:69
作者
Loos, WJ
Verweij, J
Nooter, K
Stoter, G
Sparreboom, A
机构
[1] ROTTERDAM CANC INST,DANIEL DEN HOED KLIN,DEPT MED ONCOL,LAB EXPT CHEMOTHERAPY & PHARMACOL,NL-3008 AE ROTTERDAM,NETHERLANDS
[2] UNIV ROTTERDAM HOSP,NL-3008 AE ROTTERDAM,NETHERLANDS
来源
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 693卷 / 02期
关键词
docetaxel;
D O I
10.1016/S0378-4347(97)00089-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive reversed-phase high-performance liquid chromatographic method has been developed and validated for the quantitative determination of docetaxel (I) in human plasma. The concentrations in plasma, for validation procedures spiked with known amounts of I, are read from calibration curves in the range of 10-20 000 ng/ml. The sample preparation involved a liquid-liquid extraction of 1000 mu l of sample with a mixture of acetonitrile-n-butylchloride (1:4, v/v). The related compound paclitaxel (II) was used as internal standard. Chromatographic separations were performed an Inertsil ODS-80A column, with UV detection performed at 230 nm. The overall extraction recoveries were 84.3 and 90.0% for I and II, respectively. The lower limit of quantitation was 10 ng/ml, and the accuracy, within-run and between-run precisions at three tested concentrations fell within the generally accepted criteria for bioanalytical assays.
引用
收藏
页码:437 / 441
页数:5
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