Assays for glycosyltransferases

被引：49

作者：

Palcic, MM

Sujino, K

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

[2] Raylo Chem Inc, Edmonton, AB T6C 4A9, Canada

来源：

TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY | 2001年 / 13卷 / 72期

关键词：

glycosyltransferases; radiochemical assay; continual assay; screening; inhibitors; mechanisms;

D O I：

10.4052/tigg.13.361

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A variety of methods for assaying glycosyltransferase activity have been developed driven by the specific interests and type of information required by researchers. These methods include the routine monitoring of activity during and after enzyme purification and in biocatalysis, detailed mechanistic and inhibition evaluations, and high throughput screening for novel glycosyltransferase activities or inhibitors. Representative assay methods for all of these applications including their advantages and limitations are summarized in this review.

引用

页码：361 / 370

页数：10

共 107 条

[1] AN IMPROVED ASSAY-METHOD FOR UDP-GLUCURONYLTRANSFERASE ACTIVITY TOWARDS 5-HYDROXYTRYPTAMINE AND THE CHARACTERISTIC PROPERTIES OF THE ENZYME [J].