White adipose tissue nitric oxide synthase: A potential source for NO production

被引：111

作者：

Ribiere, C

Jaubert, AM

Gaudiot, N

Sabourault, D

Marcus, ML

Boucher, JL

DenisHenriot, D

Giudicelli, Y

机构：

[1] UNIV PARIS 05,CNRS,URA 400,F-75006 PARIS,FRANCE

[2] CTR HOSP POISSY,FAC MED PARIS OUEST,INSERM,CJF 94 02,DEPT BIOCHEM,F-75006 PARIS,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 222卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.0824

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitric oxide synthase (NOS) activity was detected in soluble and membranous fractions of adipose tissue homogenates of control rats. After LPS-treatment, this activity was (i) markedly increased (about 10-fold) in both fractions, (ii) unaltered after dexamethasone pretreatment, (iii) partly calcium-calmodulin sensitive, and (iv) almost entirely accounted by the NOS activity found in isolated adipocytes. in adipose tissue homogenates from control rats, Western blot analysis demonstrated the presence of the endothelial (eNOS) isoform in the membranous fraction of control rats and of the inducible (iNOS) isoform in the soluble and membranous fractions. After LPS treatment. the amount of immunoreactive iNOS protein was dramatically increased, suggesting that adipose tissue is an important site of NO production during the endotoxic shock. (C) 1996 Academic Press, Inc.

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页码：706 / 712

页数：7

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