Monitoring of oxytetracycline in ovine milk by high-performance liquid chromatography

被引:17
作者
Boatto, G
Pau, A
Palomba, M
Arenare, L
Cerri, R
机构
[1] Univ Sassari, Fac Farm, Dipartimento Farmaco Chim Tossicol, I-07100 Sassari, Italy
[2] Univ Naples, Fac Farm, Dipartimento Chim Farmaceut & Tossicol, I-80131 Naples, Italy
关键词
oxytetracycline; in vivo monitoring; trace amounts; reversed-phase liquid chromatography; milk;
D O I
10.1016/S0731-7085(99)00050-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method for 'in vivo' determination of the oxytetracyclin residues in ovine milk at low levels is described. Two groups of Sardinian breed sheep were treated with a dose of oxytetracycline by intramammary infusion and intramuscular administration, respectively. Oxytetracycline residues in extracts obtained from a preliminary cleanup procedure, were detected by an isocratic high-performance liquid chromatography (HPLC) method. Linear calibration plots were obtained over a large concentration range of 1 mg ml(-1)-10 ng ml(-1), with correlation coefficients higher than 0.996. Recoveries between 85.8 and 98.9% were obtained. Limit of detection (LOD) and limit of determination (LOQ) were 5.2 and 17.5 ng ml(-1), respectively. This method would be useful for routine monitoring of oxytetracycline residues in ovine dairy milk. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:321 / 326
页数:6
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