Analysis of kinesin motor function at budding yeast kinetochores

被引:93
作者
Tytell, JD
Sorger, PK [1 ]
机构
[1] MIT, Dept Biol, Cambridge, MA 02139 USA
[2] MIT, Biol Engn Div, Cambridge, MA 02139 USA
关键词
D O I
10.1083/jcb.200509101
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Accurate chromosome segregation during mitosis requires biorientation of sister chromatids on the microtubules (MT) of the mitotic spindle. Chromosome-MT binding is mediated by kinetochores, which are multiprotein structures that assemble on centromeric (CEN) DNA. The simple CENs of budding yeast are among the best understood, but the roles of kinesin motor proteins at yeast kinetochores have yet to be determined, despite evidence of their importance in higher eukaryotes. We show that all four nuclear kinesins in Saccharomyces cerevisiae localize to kinetochores and function in three distinct processes. Kip1p and Cin8p, which are kinesin-5/BimC family members, cluster kinetochores into their characteristic bilobed metaphase configuration. Kip3p, a kinesin-8,-13/Kinl kinesin, synchronizes poleward kinetochore movement during anaphase A. The kinesin-14 motor Kar3p appears to function at the subset of kinetochores that become detached from spindle MTs. These data demonstrate roles for structurally diverse motors in the complex processes of chromosome segregation and reveal important similarities and intriguing differences between higher and lower eukaryotes.
引用
收藏
页码:861 / 874
页数:14
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