Rational saccharide extension by using the natural product glycosyltransferase LanGT4

被引：18

作者：

Hoffmeister, D

Weber, M

Dräger, G

Ichinose, K

Dürr, C

Bechthold, A

机构：

[1] Univ Freiburg, Inst Pharmazeut Wissensch, D-79104 Freiburg, Germany

[2] Univ Wisconsin, Sch Pharm, Madison, WI 53705 USA

[3] Leibniz Univ Hannover, Inst Organ Chem, D-30167 Hannover, Germany

[4] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

CHEMBIOCHEM | 2004年 / 5卷 / 03期

关键词：

biosynthesis; drug design; enzymes; glycosyltransferase; urdamycin;

D O I：

10.1002/cbic.200300793

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adding more sugar: Overexpression of the landomycin glycosyltransferase gene lanGT4 in Streptomyces fradiae Tü2717 resulted in the conversion of the urdamycin trisaccharide into an unnatural tetrasaccharide by transfer of an L-rhodinose unit. We propose that LanGT4 is able to act iteratively on growing saccharide chains.

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收藏

页码：369 / 371

页数：3

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