Antibacterial activity of dental composites containing quaternary ammonium polyethylenimine nanoparticles against Streptococcus mutans

The antibacterial activity of quaternary ammonium polyethylenimine (PEI) nanoparticles embedded at 1% w/w with clinically used bonding, flowable and hybrid dental composite resins and cured by light polymerization was studied. The antibacterial activity was tested with Streptoccocus mutans by: (i) the agar diffusion test (ADT); (ii) the direct contact test; (iii) bacterial growth in the materials elute; (iv) and scanning electron microscope (SEM). Using the direct contact test, antibacterial activity (p < 0.001) was found in all three types of composite resins incorporated with the synthesized nanoparticles. The effect lasted for at least I month. SEM demonstrated bacterial debris and no streptococcal chains at 24 h of bacterial contact. The addition of I% w/w of nanoparticles did not affect the flexural modulus and the flexural strength of the dental composite materials.The results indicate that quaternary ammonium PEI nanoparticles immobilized in resin-based materials have a strong antibacterial activity upon contact without leach-out of the nanoparticles and without compromise in mechanical properties. (c) 2006 Elsevier Ltd. All rights reserved.