Inhibition of terminal chondrocyte differentiation by bone morphogenetic protein 7 (OP-1) in vitro depends on the periarticular region but is independent of parathyroid hormone-related peptide

被引:41
作者
Haaijman, A
Karperien, M
Lanske, B
Hendriks, J
Löwik, CWGM
Bronckers, ALJJ
Burger, EH
机构
[1] Free Univ Amsterdam, Dept Oral Cell Biol ACTA, NL-1081 BT Amsterdam, Netherlands
[2] Leiden Univ, Med Ctr, Dept Endocrinol & Metab Dis, Leiden, Netherlands
[3] Leiden Univ, Med Ctr, Dept Pediat, Leiden, Netherlands
[4] Massachusetts Gen Hosp, Endocrine Unit, Boston, MA 02114 USA
[5] Netherlands Inst Dev Biol, Utrecht, Netherlands
关键词
bone morphogenetic protein 7 (BMP-7); parathyroid hormone (PTH)-related peptide; Indian hedgehog (Ihh); endochondral ossification; chondrocyte hypertrophy;
D O I
10.1016/S8756-3282(99)00189-1
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Bone morphogenetic protein-7, or BMP-7 (OP-l), is highly expressed in the perichondrium of embryonic long bones and is thought to play a role in endochondral ossification. Previously we have shown that BMP-7 inhibits terminal chondrocyte differentiation; that is, chondrocyte hypertrophy and mineralization in cultured explants of embryonic mouse metatarsals. However, the mechanism of this inhibition and the target cells of BMP-7 are still unknown, In this study we show that BMP-7 inhibits terminal chondrocyte differentiation indirectly, via an interaction with the periarticular region of the explants, This region also expresses parathyroid hormone-related peptide (PTHrP), PTHrP regulates terminal chondrocyte differentiation by inhibiting hypertrophic differentiation of prehypertrophic chondrocytes, The differentiating center in turn regulates PTHrP expression via a feedback loop involving Indian hedgehog (Ihh), which is expressed in the prehypertrophic chondrocytes, Ihh is thought to act on perichondrial cells, which in turn start to express an as yet unknown mediator that stimulates PTHrP expression in the periarticular region, It has been suggested that this factor belongs to the BMP-family, We investigated whether the inhibition of terminal chondrocyte differentiation by BMP-7 was due to upregulation of the PTHrP-Ihh feedback loop and whether BMP-7 was the unknown factor in the loop. Here we show that exogeneous BMF-7 did not upregulate the mRNA expression of PTHrP, Ihh, or the PTH/PTHrP receptor in cultured wild-type embryonic metatarsals, Furthermore, BMP-7 could still inhibit terminal chondrocyte differentiation in the metatarsals of PTHrP-deficient (PTHrP-/-) mouse embryos, These data indicate that the BMP-7-mediated inhibition of terminal chondrocyte differentiation in vitro is independent of the PTHrP-Ihh feedback loop. We concluded that BMP-7 modulates terminal chondrocyte differentiation and cartilage mineralization of fetal bone explants in vitro via as yet unknown inhibitory factor(s) produced in the periarticular region, (C) 1999 by Elsevier Science Inc. All rights reserved.
引用
收藏
页码:397 / 404
页数:8
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