T cell receptor excision circles as markers for recent thymic emigrants: basic aspects, technical approach, and guidelines for interpretation

被引:245
作者
Hazenberg, MD
Verschuren, MCM
Hamann, D
Miedema, F
van Dongen, JJM
机构
[1] Univ Amsterdam, Acad Med Ctr, Dept Clin Viroimmunol, CLB, NL-1066 CX Amsterdam, Netherlands
[2] Univ Amsterdam, Acad Med Ctr, Expt & Clin Immunol Lab, NL-1066 CX Amsterdam, Netherlands
[3] Erasmus Univ, Univ Rotterdam Hosp, Dept Immunol, Rotterdam, Netherlands
来源
JOURNAL OF MOLECULAR MEDICINE-JMM | 2001年 / 79卷 / 11期
关键词
TREC; thymus; T cell receptor gene rearrangements; human immunodeficiency virus; reconstitution;
D O I
10.1007/s001090100271
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
T cell differentiation in the thymus is characterized by a hierarchical order of rearrangement steps' in the T cell receptor (TCR) genes, resulting in the joining of V, D, and J gene segments. During each of the rearrangement steps, DNA fragments between rearranging V, D. and J gene segments are deleted as circular excision products, the so-called TRECs (T cell receptor excision circles). TRECs are assumed to have a high over-time stability, but they can not multiply and consequently are diluted during T cell proliferation. It was recently suggested that quantitative detection of TRECs would allow for direct measurement of thymic output. The delta Rec-psiJ alpha TREC appears to be the best marker, because the majority of thymocyte expansion occurs before this TREC is formed. However, apart from thymic output several other factors determine the TREC content of a T cell population, such as cell division and cell death. Likewise, the number of TRECs depends not only on thymic output, but also on the longevity of naive T cells. This warrants caution with regard to the interpretation of TREC data as measured in healthy and diseased individuals. delta Rec-psiJ alpha TREC detection is a new and elegant tool for identification of recent thymic emigrants in the periphery, but further research is required for making quantitative estimations of thymic output with the use of TREC analysis.
引用
收藏
页码:631 / 640
页数:10
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