AFM-based Force Spectroscopy on Polystyrene Brushes: Effect of Brush Thickness on Protein Adsorption

被引：20

作者：

Hentschel, Carsten ^{[1
,2
]}

Wagner, Hendrik ^{[3
]}

Smiatek, Jens ^{[4
]}

Heuer, Andreas ^{[4
]}

Fuchs, Harald ^{[1
,2
]}

Zhang, Xi ^{[5
]}

Studer, Armido ^{[3
]}

Chi, Lifeng ^{[1
,2
]}

机构：

[1] Univ Munster, Inst Phys, D-48149 Munster, Germany

[2] Ctr Nanotechnol CeNTech, D-48149 Munster, Germany

[3] Univ Munster, Inst Organ Chem, D-48149 Munster, Germany

[4] Univ Munster, Inst Phys Chem, D-48149 Munster, Germany

[5] Tsinghua Univ, Dept Chem, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China

来源：

LANGMUIR | 2013年 / 29卷 / 06期

关键词：

DISSIPATIVE PARTICLE DYNAMICS; SELF-ASSEMBLED MONOLAYERS; SINGLE-MOLECULE; POLYMER BRUSHES; SURFACE MODIFICATION; IMMOBILIZATION; DERIVATIVES; MICROARRAYS; MICROSCOPY; STRATEGIES;

D O I：

10.1021/la302212h

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Herein we present a study on nonspecific binding of proteins at highly dense packed hydrophobic polystyrene brushes. In this context, an atomic force microscopy tip was functionalized with concanavalin A to perform single-molecule force spectroscopy measurements on polystyrene brushes with thicknesses of 10 and 60 nm, respectively. Polystyrene brushes with thickness of 10 nm show an almost two times stronger protein adsorption than brushes with a thickness of 60 rim: 72 pN for the thinner and 38 pN for the thicker layer, which is in qualitative agreement with protein adsorption studies conducted macroscopically by fluorescence microscopy.

引用

页码：1850 / 1856

页数：7

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