Characterization of heavy metal-resistant endophytic bacteria from rape (Brassica napus) roots and their potential in promoting the growth and lead accumulation of rape

被引：463

作者：

Sheng, Xia-Fang ^{[1
]}

Xia, Juan-Juan ^{[1
]}

Jiang, Chun-Yu ^{[1
]}

He, Lin-Yan ^{[1
]}

Qian, Meng ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Life Sci, MOA Key Lab Microbiol Engn Agr Environm, Nanjing 210095, Peoples R China

来源：

ENVIRONMENTAL POLLUTION | 2008年 / 156卷 / 03期

关键词：

Heavy metal-resistant endophytic bacteria; Plant growth promotion; IAA; Siderophore; ACC; Rape; Phytoextraction;

D O I：

10.1016/j.envpol.2008.04.007

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Two lead (Pb)-resistant endophytic bacteria were isolated from rape roots grown in heavy metal-contaminated soils and characterized. A pot experiment was conducted for investigating the capability of the two isolates to promote the growth and Pb uptake of rape from Pb-amended soil. The two isolates were identified as Pseudomonas fluorescens G10 and Microbacterium sp. G16 based on the 16S rDNA gene sequence analysis. Strains G10 and G16 exhibited different multiple heavy metal and antibiotic resistance characteristics and increased water-soluble Pb in solution and in Pb-added soil. Root elongation assays demonstrated increases in root elongation of inoculated rape seedlings compared to the control plants. Strain G16 produced indole acetic acid, siderophores and 1-aminocyclopropane-1-carboxylate deaminase. Increases in biomass production and total Pb uptake in the bacteria-inoculated plants were obtained compared to the control. The two strains could colonize the root interior and rhizosphere soil of rape after root inoculation. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1164 / 1170

页数：7

共 42 条

[1] Bacterial inoculants affecting nickel uptake by Alyssum murale from low, moderate and high Ni soils [J].