RNA in forensic science

被引:135
作者
Bauer, M. [1 ]
机构
[1] Univ Bonn, Inst Legal Med, D-53111 Bonn, Germany
关键词
RNA; Wound age determination; Real time PCR; Body fluid identification; Gene expression;
D O I
10.1016/j.fsigen.2006.11.002
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
RNA analysis offers insight into diseases and mechanisms leading to death and could develop into a valuable tool for diagnosis of the cause of death in forensic pathology. Other possible applications include the determination of the age of wounds and injuries and of the post-mortem interval. The molecular identification of body fluids by analysis of cell-specific mRNA expression already represents a new technique supplementing DNA analysis in forensic cases. This review explains and discusses principles, techniques and applications by offering a complete and comprehensive overview of research results in forensic RNA work. (C) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:69 / 74
页数:6
相关论文
共 54 条
[1]   An mRNA and DNA co-isolation method for forensic casework samples [J].
Alvarez, M ;
Juusola, J ;
Ballantyne, J .
ANALYTICAL BIOCHEMISTRY, 2004, 335 (02) :289-298
[2]   A method for determining the age of a bloodstain [J].
Anderson, S ;
Howard, B ;
Hobbs, GR ;
Bishop, CP .
FORENSIC SCIENCE INTERNATIONAL, 2005, 148 (01) :37-45
[3]   Gene expression profiling in the post-mortem human brain - no cause for dismay [J].
Bahn, S ;
Augood, S ;
Standaert, DG ;
Starkey, M ;
Emson, PC .
JOURNAL OF CHEMICAL NEUROANATOMY, 2001, 22 (1-2) :79-94
[4]   TaqMan PCR assay in the control of RNA normalization in human post-mortem brain tissue [J].
Barrachina, Marta ;
Castano, Esther ;
Ferrer, Isidro .
NEUROCHEMISTRY INTERNATIONAL, 2006, 49 (03) :276-284
[5]   PREMORTEM AND POSTMORTEM INFLUENCES ON BRAIN-RNA [J].
BARTON, AJL ;
PEARSON, RCA ;
NAJLERAHIM, A ;
HARRISON, PJ .
JOURNAL OF NEUROCHEMISTRY, 1993, 61 (01) :1-11
[6]   Quantification of RNA degradation by semi-quantitative duplex and competitive RT-PCR: a possible indicator of the age of bloodstains? [J].
Bauer, M ;
Polzin, S ;
Patzelt, D .
FORENSIC SCIENCE INTERNATIONAL, 2003, 138 (1-3) :94-103
[7]   A method for simultaneous RNA and DNA isolation from dried blood and semen stains [J].
Bauer, M ;
Patzelt, D .
FORENSIC SCIENCE INTERNATIONAL, 2003, 136 (1-3) :76-78
[8]   Protamine mRNA as molecular marker for spermatozoa in semen stains [J].
Bauer, M ;
Patzelt, D .
INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 2003, 117 (03) :175-179
[9]  
Bauer M, 2002, J FORENSIC SCI, V47, P1278
[10]  
Bauer M, 1999, J FORENSIC SCI, V44, P1232