Functional binding of a vertebrate hormone, L-3,5,3′-triiodothyronine (T3), on insect follicle cell membranes

被引：32

作者：

Kim, Y ^{[1
]}

Davari, ED

Sevala, V

Davey, KG

机构：

[1] Andong Natl Univ, Sch Bioresource Sci, Andong 760749, South Korea

[2] York Univ, Dept Biol, N York, ON M3J 1P3, Canada

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1999年 / 29卷 / 10期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Locusta migratoria; Rhodnius prolixus; follicle cell; L-3,5,3 '-triiodothyronine; juvenile hormone; membrane receptor;

D O I：

10.1016/S0965-1748(99)00070-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A vertebrate hormone, L-3,5,3'-triiodothyronine (T-3), induces volume reduction in the follicle cells of Locusta migratoria and Rhodnius prolixus. The effect of T-3 on locust follicle cells is inhibited by ouabain and by antibodies raised against a membrane binding protein for juvenile hormone (JH). [I-125]-T-3 binds to membrane preparations of vitellogenic follicles in a specific and saturable fashion, with a K-D in the low nanomolar range. T-3 and JH III exhibited equivalent abilities to compete for the T-3 binding site. These findings strongly suggest that T-3 and JH act via the same receptor in follicle cells. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：943 / 950

页数：8

共 35 条

[1]

ABUHAKIMA R, 1977, J EXP BIOL, V69, P33

[2] EFFECTS OF SOME VERTEBRATE HORMONES ON LIPID MOBILIZATION IN INSECT FAT BODY [J].