Single molecule study of the reaction between DNA and formamide

被引：7

作者：

Bhattacharyya, AJ

Feingold, M ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Ilse Katz Ctr Meso & Nanoscale Sci & Technol, IL-84105 Beer Sheva, Israel

来源：

TALANTA | 2001年 / 55卷 / 05期

关键词：

single molecule; DNA stretching; optical tweezers; formamide;

D O I：

10.1016/S0039-9140(01)00493-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The kinetics of the reaction between double stranded DNA (dsDNA) and formamide is monitored at the single DNA molecule level. We find that stretching of the DNA leads to an accelerated reaction rate and to a shift in the final equilibrium concentrations. The larger the stretching force, the faster the reaction and the larger the denatured fraction of the product DNA. The single molecule kinetics is obtained from the change in the contour length of the DNA which, in turn, is measured using optical tweezers on a microbead-single DNA molecule-cover slip construct. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：943 / 949

页数：7

共 36 条

[1]

Alberts B., 1994, MOL BIOL CELL

[2] pH-dependent specific binding and combing of DNA [J].