Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli

被引：108

作者：

Mukougawa, K

Kanamoto, H

Kobayashi, T

Yokota, A

Kohchi, T ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Biostudies, Sakyo Ku, Kyoto 6068502, Japan

[2] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300192, Japan

来源：

FEBS LETTERS | 2006年 / 580卷 / 05期

基金：

日本学术振兴会;

关键词：

bilin reductase; chromophore; phycocyanobilin; phycoerythrobilin; phytochrome; phytochromobilin;

D O I：

10.1016/j.febslet.2006.01.051

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By co-expression of heme oxygenase and various bilin reductase(s) in a single operon in conjunction with apophytochrome using two compatible plasmids, we developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were assembled with phytochromobilin, phycocyanobilin, and phycocrythrobilin. The blue-shifted difference spectra of truncated phytochromes were observed with a phycocyanobilin chromophore compared to a phytochromobilin chromophore. When the phycoerythrobilin biosynthetic enzymes were co-expressed, E. coli cells accumulated orange-fluorescent phytochrome. The metabolic engineering of bacteria for the production of various bilins for assembly into phytochromes will facilitate the molecular analysis of photoreceptors. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1333 / 1338

页数：6

共 29 条

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