TiO2 Nanoparticle Exposure and Illumination during Zebrafish Development: Mortality at Parts per Billion Concentrations

被引:87
作者
Bar-Ilan, Ofek [1 ]
Chuang, Connie C. [1 ]
Schwahn, Denise J. [2 ]
Yang, Sarah [3 ]
Joshi, Sanjay [6 ]
Pedersen, Joel A. [3 ,4 ]
Hamers, Robert J. [5 ]
Peterson, Richard E. [1 ,3 ]
Heideman, Warren [1 ,3 ]
机构
[1] Univ Wisconsin, Sch Pharm, Div Pharmaceut Sci, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Chem, Res Anim Resources Ctr, Madison, WI 53706 USA
[3] Univ Wisconsin, Mol & Environm Toxicol Ctr, Madison, WI 53706 USA
[4] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA
[5] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA
[6] Penn State Univ, Dept Ind & Mfg Engn, University Pk, PA 16802 USA
基金
美国国家科学基金会;
关键词
OXIDATIVE DAMAGE; NANOMATERIALS;
D O I
10.1021/es304514r
中图分类号
X [环境科学、安全科学];
学科分类号
083001 [环境科学];
摘要
Photoactivation of titanium dioxide nanoparticles (TiO(2)NPs) can produce reactive oxygen species (ROS). Over time, this has the potential to produce cumulative cellular damage. To test this, we exposed zebrafish (Danio rerio) to two commercial TiO2NP preparations at concentrations ranging from 0.01 to 10 000 ng/mL over a 23 day period spanning embryogenesis, larval development, and juvenile metamorphosis. Fish were illuminated with a lamp that mimics solar irradiation. TiO2NP exposure produced significant mortality at 1 ng/mL. Toxicity included stunted growth, delayed metamorphosis, malformations, organ pathology, and DNA damage. TiO(2)NPs were found in the gills and gut and elsewhere. The two preparations differed in nominal particle diameter (12.1 +/- 3.7 and 23.3 +/- 9.8 nm) but produced aggregates in the 1 mu m range. Both were taken up in a dose-dependent manner. Illuminated particles produced a time- and dose-dependent increase in 8-hydroxy-2'-deoxyguanosine DNA adducts consistent with cumulative ROS damage. Zebrafish take up TiO(2)NPs from the aqueous environment even at low ng/mL concentrations, and these particles when illuminated in the violet-near UV range produce cumulative toxicity.
引用
收藏
页码:4726 / 4733
页数:8
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