Development of Miniaturized Walking Biological Machines

被引：185

作者：

Chan, Vincent ^{[1
,5
]}

Park, Kidong ^{[2
,5
]}

Collens, Mitchell B. ^{[1
,5
]}

Kong, Hyunjoon ^{[3
]}

Saif, Taher A. ^{[4
]}

Bashir, Rashid ^{[1
,2
,5
]}

机构：

[1] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Elect & Comp Engn, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Mech Sci & Engn, Urbana, IL 61801 USA

[5] Univ Illinois, Micro & Nanotechnol Lab, Urbana, IL 61801 USA

来源：

SCIENTIFIC REPORTS | 2012年 / 2卷

基金：

美国国家科学基金会;

关键词：

IN-VITRO; OPTOGENETIC CONTROL; STEREOLITHOGRAPHY; HYDROGELS; MICROENVIRONMENTS; ALIGNMENT; CELLS; MODEL;

D O I：

10.1038/srep00857

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The quest to 'forward-engineer' and fabricate biological machines remains a grand challenge. Towards this end, we have fabricated locomotive "bio-bots'' from hydrogels and cardiomyocytes using a 3D printer. The multi-material bio-bot consisted of a 'biological bimorph' cantilever structure as the actuator to power the bio-bot, and a base structure to define the asymmetric shape for locomotion. The cantilever structure was seeded with a sheet of contractile cardiomyocytes. We evaluated the locomotive mechanisms of several designs of bio-bots by changing the cantilever thickness. The bio-bot that demonstrated the most efficient mechanism of locomotion maximized the use of contractile forces for overcoming friction of the supporting leg, while preventing backward movement of the actuating leg upon relaxation. The maximum recorded velocity of the bio-bot was similar to 236 mu m s(-1), with an average displacement per power stroke of similar to 354 mu m and average beating frequency of similar to 1.5 Hz.

引用

页数：8

共 44 条

[1] Stereolithography of three-dimensional bioactive poly(ethylene glycol) constructs with encapsulated cells [J].

Arcaute, Karina ;

Mann, Brenda K. ;

Wicker, Ryan B. .

ANNALS OF BIOMEDICAL ENGINEERING, 2006, 34 (09) :1429-1441

[2] Stereolithography of spatially controlled multi-material bioactive poly(ethylene glycol) scaffolds [J].

Arcaute, Karina ;

Mann, Brenda ;

Wicker, Ryan .

ACTA BIOMATERIALIA, 2010, 6 (03) :1047-1054

[3] Optogenetic Control of Cardiac Function [J].

Arrenberg, Aristides B. ;

Stainier, Didier Y. R. ;

Baier, Herwig ;

Huisken, Jan .

SCIENCE, 2010, 330 (6006) :971-974

[4] Optically controlled contraction of photosensitive skeletal muscle cells [J].

Asano, Toshifumi ;

Ishizua, Toru ;

Yawo, Hiromu .

BIOTECHNOLOGY AND BIOENGINEERING, 2012, 109 (01) :199-204

[5] A synthetic multicellular system for programmed pattern formation [J].

Basu, S ;

Gerchman, Y ;

Collins, CH ;

Arnold, FH ;

Weiss, R .

NATURE, 2005, 434 (7037) :1130-1134

[6] CONTINUAL ELECTRIC-FIELD STIMULATION PRESERVES CONTRACTILE FUNCTION OF ADULT VENTRICULAR MYOCYTES IN PRIMARY CULTURE [J].

BERGER, HJ ;

PRASAD, SK ;

DAVIDOFF, AJ ;

PIMENTAL, D ;

ELLINGSEN, O ;

MARSH, JD ;

SMITH, TW ;

KELLY, RA .

AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 266 (01) :H341-H349

[7] Influence of Substrate Stiffness on the Phenotype of Heart Cells [J].

Bhana, Bashir ;

Iyer, Rohin K. ;

Chen, Wen Li Kelly ;

Zhao, Ruogang ;

Sider, Krista L. ;

Likhitpanichkul, Morakot ;

Simmons, Craig A. ;

Radisic, Milica .

BIOTECHNOLOGY AND BIOENGINEERING, 2010, 105 (06) :1148-1160

[8] Optogenetic control of heart muscle in vitro and in vivo [J].

Bruegmann, Tobias ;

Malan, Daniela ;

Hesse, Michael ;

Beiert, Thomas ;

Fuegemann, Christopher J. ;

Fleischmann, Bernd K. ;

Sasse, Philipp .

NATURE METHODS, 2010, 7 (11) :897-U45

[9] Building off-the-shelf tissue-engineered composites [J].

Burg, Timothy ;

Cass, Cheryl A. P. ;

Groff, Richard ;

Pepper, Matthew ;

Burg, Karen J. L. .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 2010, 368 (1917) :1839-1862

[10] Cardiac muscle tissue engineering: toward an in vitro model for electrophysiological studies [J].

Bursac, N ;

Papadaki, M ;

Cohen, RJ ;

Schoen, FJ ;

Eisenberg, SR ;

Carrier, R ;

Vunjak-Novakovic, G ;

Freed, LE .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1999, 277 (02) :H433-H444

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