An upstream positive regulatory element in human GM-CSF promoter is recognized by NF-kappa B/Rel family members

被引：18

作者：

Musso, M ^{[1
]}

Ghiorzo, P ^{[1
]}

Fiorentini, P ^{[1
]}

Giuffrida, R ^{[1
]}

Ciotti, P ^{[1
]}

Garre, C ^{[1
]}

Ravazzolo, R ^{[1
]}

BianchiScarra, G ^{[1
]}

机构：

[1] UNIV GENOA,INST BIOL & GENET,IBIG,I-16132 GENOA,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 223卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.0847

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulation, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-lymphoid Mo cell line, which constitutively expresses GM-CSF, indicated that the A element, located between -2002 and -1984, has a positive role on transcription. Further characterization by electrophoretic mobility shift assays in the presence of different competitor oligonucleotides showed that this element binds a factor of the NF-kappa B/Rel family. (C) 1996 Academic Press, Inc.

引用

页码：64 / 72

页数：9

共 28 条

[1] HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I TAX INDUCES EXPRESSION OF THE REL-RELATED FAMILY OF KAPPA-B ENHANCER-BINDING PROTEINS - EVIDENCE FOR A PRETRANSLATIONAL COMPONENT OF REGULATION [J].