Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells

被引:515
作者
Relaix, F
Montarras, D
Zaffran, S
Gayraud-Morel, B
Rocancourt, D
Tajbakhsh, S
Mansouri, A
Cumano, A
Buckingham, M [1 ]
机构
[1] Inst Pasteur, INSERM, U277, Dept Immunol,Unite Genet Mol Dev, F-75724 Paris 15, France
[2] Inst Pasteur, INSERM, U277,URA 2578, Dept Biol Dev,Grp Cellules Souches & Dev,CNRS, F-75724 Paris 15, France
[3] Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany
关键词
D O I
10.1083/jcb.200508044
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 and -7 repress MyoD, but do not interfere with the expression of the other myogenic determination factor, Myf5, which, together with Pax3/7, regulates the myogenic differentiation of these cells. In Pax7 mutants, satellite cells are progressively lost in both Pax3-expressing and -nonexpressing muscles. We show that this is caused by satellite cell death, with effects on the cell cycle. Manipulation of the dominant-negative forms of these factors in satellite cell cultures demonstrates that Pax3 cannot replace the antiapoptotic function of Pax7. These findings underline the importance of cell survival in controlling the stem cell populations of adult tissues and demonstrate a role for upstream factors in this context.
引用
收藏
页码:91 / 102
页数:12
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