Mapping of alkaline proteins in bovine skeletal muscle

被引:33
作者
Chaze, T [1 ]
Bouley, J [1 ]
Chambon, C [1 ]
Barboiron, C [1 ]
Picard, B [1 ]
机构
[1] INRA, Herbivore Res Unit, Muscle Growth & Metab Grp, F-63122 St Genes Champanelle, France
关键词
alkaline proteins; skeletal muscle; troponin T; two-dimensional gel electrophoresis;
D O I
10.1002/pmic.200500452
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In agricultural sciences, proteomics has become the new hope for analyzing the meat quality traits that are closely related to the skeletal muscle traits. 2-DE muscle maps of many species have been recently reported and used to find molecular markers of meat quality traits. However, one limitation of 2-DE based analyses is due to the limited alkaline protein separation. Considering this problem, there is a need to use recent advances that have markedly improved the 2-DE based analysis of alkaline proteins. Hence, the present study provides additional information concerning the alkaline proteome of bovine skeletal muscle by using an appropriate protocol to characterize proteins over the entire range of pH 7-11. A total of 32 distinct gene products corresponding to 60 protein spots were identified by PMF and grouped in seven categories according to their main function. This 2-D map will contribute to muscle proteome studies since a significant portion of proteins is in the alkaline PH range.
引用
收藏
页码:2571 / 2575
页数:5
相关论文
共 18 条
[1]   The LIM domain: regulation by association [J].
Bach, I .
MECHANISMS OF DEVELOPMENT, 2000, 91 (1-2) :5-17
[2]   Calcium ion in skeletal muscle:: Its crucial role for muscle function, plasticity, and disease [J].
Berchtold, MW ;
Brinkmeier, H ;
Müntener, M .
PHYSIOLOGICAL REVIEWS, 2000, 80 (03) :1215-1265
[3]   Proteomic analysis of bovine skeletal muscle hypertrophy [J].
Bouley, J ;
Meunier, B ;
Chambon, C ;
De Smet, S ;
Hocquette, JF ;
Picard, B .
PROTEOMICS, 2005, 5 (02) :490-500
[4]   Mapping of bovine skeletal muscle proteins using two-dimensional gel electrophoresis and mass spectrometry [J].
Bouley, J ;
Chambon, C ;
Picard, B .
PROTEOMICS, 2004, 4 (06) :1811-1824
[5]   Improved resolution of the human adipose tissue proteome at alkaline and wide range pH by the addition of hydroxyethyl disulfide [J].
Cortón, M ;
Villuendas, G ;
Botella, JI ;
San Millán, JL ;
Escobar-Morreale, HF ;
Peral, B .
PROTEOMICS, 2004, 4 (02) :438-441
[6]   Current two-dimensional electrophoresis technology for proteomics [J].
Görg, A ;
Weiss, W ;
Dunn, MJ .
PROTEOMICS, 2004, 4 (12) :3665-3685
[7]  
Hoving S, 2002, PROTEOMICS, V2, P127, DOI 10.1002/1615-9861(200202)2:2<127::AID-PROT127>3.0.CO
[8]  
2-Y
[9]   Analyzing alkaline proteins in human colon crypt proteome [J].
Li, XM ;
Patel, BB ;
Blagoi, EL ;
Patterson, MD ;
Seehozer, SH ;
Zhang, T ;
Damle, S ;
Gao, ZQ ;
Boman, B ;
Yeung, AT .
JOURNAL OF PROTEOME RESEARCH, 2004, 3 (04) :821-833
[10]   30,000-DALTON COMPONENT OF TENDER BOVINE LONGISSIMUS MUSCLE [J].
MACBRIDE, MA ;
PARRISH, FC .
JOURNAL OF FOOD SCIENCE, 1977, 42 (06) :1627-1629