Reproducible Comet assay of amorphous silica nanoparticles detects no genotoxicity

被引：167

作者：

Barnes, Clifford A. ^{[3
]}

Elsaesser, Andreas ^{[3
]}

Arkusz, Joanna ^{[2
]}

Smok, Anna ^{[2
]}

Palus, Jadwiga ^{[2
]}

Lesniak, Anna ^{[1
]}

Salvati, Anna ^{[1
]}

Hanrahan, John P. ^{[4
]}

de Jong, Wirn H. ^{[5
]}

Dziubaltowska, Elzbieta ^{[2
]}

Stepnik, Maciej ^{[2
]}

Rydzynski, Konrad ^{[2
]}

McKerr, George ^{[3
]}

Lynch, Iseult ^{[1
]}

Dawson, Kenneth A. ^{[1
]}

Howard, C. Vyvyan ^{[3
]}

机构：

[1] Univ Coll Dublin, Ctr BioNano Interact, Dublin 4, Ireland

[2] Nofer Inst Occupat Med, Lodz, Poland

[3] Univ Ulster, Coleraine BT52 1SA, Londonderry, North Ireland

[4] Glantreo Ltd, Rubicon Ctr, Cork, Ireland

[5] Natl Inst Publ Hlth & Environm, NL-3720 BA Bilthoven, Netherlands

来源：

NANO LETTERS | 2008年 / 8卷 / 09期

关键词：

D O I：

10.1021/nl801661w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By using a carefully developed protocol and careful characterization of the nanoparticle dispersions, Comet assays were performed on 3T3-L1 fibroblasts with 3, 6, and 24 h incubations and 4 or 40 mu g/ml of silica nanoparticles. No significant genotoxicity was observed for the nanoparticles tested under the conditions described, and results were independently validated in two separate laboratories, showing that in vitro toxicity testing can be quantitatively reproducible.

引用

页码：3069 / 3074

页数：6

共 20 条

[1] In vitro cytotoxicity of oxide nanoparticles: Comparison to asbestos, silica, and the effect of particle solubility [J].

Brunner, Tobias J. ;

Wick, Peter ;

Manser, Pius ;

Spohn, Philipp ;

Grass, Robert N. ;

Limbach, Ludwig K. ;

Bruinink, Arie ;

Stark, Wendelin J. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2006, 40 (14) :4374-4381

[2] In vitro genotoxicity assessment of commercial quartz flours in comparison to standard DQ12 quartz [J].

Cakmak, GD ;

Schins, RPF ;

Shi, TM ;

Fenoglio, I ;

Fubini, B ;

Borm, PJA .

INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, 2004, 207 (02) :105-113

[3] Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles [J].

Cedervall, Tommy ;

Lynch, Iseult ;

Lindman, Stina ;

Berggard, Tord ;

Thulin, Eva ;

Nilsson, Hanna ;

Dawson, Kenneth A. ;

Linse, Sara .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (07) :2050-2055

[4] In vitro cytotoxicitiy of silica nanoparticles at high concentrations strongly depends on the metabolic activity type of the cell line [J].

Chang, Jenq-Sheng ;

Chang, Ke Liang B. ;

Hwang, Deng-Fwu ;

Kong, Zwe-Ling .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2007, 41 (06) :2064-2068

[5] The potential environmental impact of engineered nanomaterials [J].

Colvin, VL .

NATURE BIOTECHNOLOGY, 2003, 21 (10) :1166-1170

[6] Cytotoxicity and DNA-damage in human lung epithelial cells exposed to respirable α-quartz [J].

Fanizza, Carla ;

Ursini, Cinzia Lucia ;

Paba, Emilia ;

Ciervo, Aureliano ;

Di Francesco, Arlanna ;

Maiello, Raffaele ;

De Simone, Paolo ;

Cavallo, Delia .

TOXICOLOGY IN VITRO, 2007, 21 (04) :586-594

[7] Silicosis: A review [J].

Greenberg, Michael I. ;

Waksman, Javier ;

Curtis, John .

DM DISEASE-A-MONTH, 2007, 53 (08) :394-416

[8] Untitled [J].

Gulledge, William P. .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2007, 634 (1-2) :241-242

[9] Toxicity of luminescent silica nanoparticles to living cells [J].

Jin, Yuhui ;

Kannan, Shibichakravarthy ;

Wu, Min ;

Zhao, Julia Xiaojun .

CHEMICAL RESEARCH IN TOXICOLOGY, 2007, 20 (08) :1126-1133

[10] In vitro toxicity of silica nanoparticles in human lung cancer cells [J].

Lin, Weisheng ;

Huang, Yue-wern ;

Zhou, Xiao-Dong ;

Ma, Yinfa .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2006, 217 (03) :252-259

← 1 2 →