9,13-di-cis-retinoic acid induces the production of tPA and activation of latent TGF-beta via RAR alpha in a human liver stellate cell line, LI90

被引：48

作者：

Imai, S

Okuno, M

Moriwaki, H

Muto, Y

Murakami, K

Shudo, K

Suzuki, Y

Kojima, S

机构：

[1] RIKEN,INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,LAB GENE TECHNOL & SAFETY,TSUKUBA,IBARAKI 305,JAPAN

[2] GIFU UNIV,SCH MED,DEPT INTERNAL MED 1,GIFU 500,JAPAN

[3] TOHOKU UNIV,SCH MED,DEPT PATHOL 1,AOBA KU,SENDAI,MIYAGI 980,JAPAN

[4] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

FEBS LETTERS | 1997年 / 411卷 / 01期

关键词：

9,13-di-cis-retinoic acid; RAR alpha; tPA; TCF-beta; proteolytic activation; liver stellate cell;

D O I：

10.1016/S0014-5793(97)00673-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We studied the mechanism by which 9,13-di-cis-retinoic acid (9,13dcRA), a novel and endogenous stereoisomer of all-trans-RA, induces TGF-beta formation in a human liver stellate cell line, LI90. 9,13dcRA induced the expression of RAR alpha and RAR beta, enhanced the production of tissue-type plasminogen activator (tPA), thereby, surface plasmin levels, and induced the activation of latent TGF-beta. Similar effects were obtained with RAR alpha-selective retinoid, but not with RAR beta- or RAR gamma-selective retinoid, and the induction was inhibited by RAR alpha-selective antagonist. These results suggest that 9,13dcRA up-regulates tPA expression, resulting in the formation of TGP-beta by LI90 cells, at least in part, via induction and activation of RAR alpha. (C) 1997 Federation of European Biochemical Societies.

引用

页码：102 / 106

页数：5

共 28 条

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