The structure of the O-polysaccharide of the Pseudoalteromonas rubra ATCC 29570T lipopolysaccharide containing a keto sugar

被引：23

作者：

Kilcoyne, M

Shashkov, AS

Knirel, YA

Gorshkova, RP

Nazarenko, EL

Ivanova, EP

Gorshkova, NM

Senchenkova, SN

Savage, AV ^{[1
]}

机构：

[1] Natl Univ Ireland, Dept Chem, Galway, Ireland

[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia

[3] Russian Acad Sci, Pacific Inst Bioorgan Chem, Far E Branch, Vladivostok 690022, Russia

来源：

CARBOHYDRATE RESEARCH | 2005年 / 340卷 / 15期

基金：

英国惠康基金;

关键词：

Pseudoalteromonas rubra; polysaccharide; NMR spectroscopy; 2-acetamido-2,6-dideoxy-D-xylo-hexos-4-ulose; malic acid;

D O I：

10.1016/j.carres.2005.07.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The structure of the phenol-soluble polysaccharide from Pseudoalteromonas rubra type strain ATCC 29570(T) has been elucidated using H-1 and C-13 NMR spectroscopy, including 2D COSY, TOCSY, gNOESY, ROESY, H-1, C-13 gHMQC and gHMBC experiments. It is concluded that the trisaccharide repeating unit of the polysaccharide has the following structure: -->4)-alpha-L-GalpNAm3AcA-(1-->3)-alpha-Sugp-(1-->4)-beta-D-GlcpNAc3NAcylA-(1--> where Sug is 2-acetamido-2,6-dideoxy-D-xylo-hexos-4-ulose, Am is acetirnidoyl and Acyl is a malic acid residue, which is O-acetylated in similar to 70% of the units. (C) 2005 Elsevier Ltd. All rights reserved.

引用

页码：2369 / 2375

页数：7

共 27 条

[1]

Genetic locus and structural characterization of the biochemical defect in the O-antigenic polysaccharide of the symbiotically deficient Rhizobium etli mutant, CE166 -: Replacement of N-acetylquinovosamine with its hexosyl-4-ulose precursor [J].