Identification of Bartonella-specific immunodominant antigens recognized by the feline humoral immune system

被引:30
作者
Freeland, RL
Scholl, DT
Rohde, KR
Shelton, LJ
O'Reilly, KL
机构
[1] Louisiana State Univ, Sch Vet Med, Dept Vet Microbiol & Parasitol, Baton Rouge, LA 70803 USA
[2] Louisiana State Univ, Sch Vet Med, Dept Epidemiol & Community Hlth, Baton Rouge, LA 70803 USA
关键词
D O I
10.1128/CDLI.6.4.558-566.1999
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The seroreactivities of both naturally and experimentally infected cats to Bartonella henselae was examined. Serum samples collected weekly from nine cats experimentally infected with B. henselae LSU16 were tested by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. The magnitude and isotype of the antibody response were investigated by ELISA, Western blot analysis allowed the identification of at least 24 Bartonella-specific antigens recognized by the cats during infection. Antibody titers to specific antigens, as determined by Western blot analysis, ranged from 10 to 640 and varied among the different antibody-antigen interactions. Absorption of sera from an experimentally infected cat, using whole cells and cell lysates of various Bartonella species and other bacteria that commonly colonize cats, supported the identification of those Bartonella-specific antigens recognized by the experimentally infected cats. Furthermore, a number of possible species- and type-specific antigens were identified. Finally, sera obtained from cats at local animal shelters were screened for the presence of antibodies directed against the Bartonella-specific bands identified in the experimentally infected cats. A number of Bartonella-specific antigens have been identified to which strong antibody responses are generated in both experimentally and naturally infected cats, some of which may be useful in diagnosing species- and/or type-specific infections. In addition, the results from these experiments will lead to the development of monoclonal antibodies targeted against those genus-, species-, and type-specific antigens.
引用
收藏
页码:558 / 566
页数:9
相关论文
共 44 条
[1]   Experimental and natural infection with Bartonella henselae in domestic cats [J].
Abbott, RC ;
Chomel, BB ;
Kasten, RW ;
FloydHawkins, KA ;
Kikuchi, Y ;
Koehler, JE ;
Pedersen, NC .
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 1997, 20 (01) :41-51
[2]   CAT-SCRATCH DISEASE, BACILLARY ANGIOMATOSIS, AND OTHER INFECTIONS DUE TO ROCHALIMAEA [J].
ADAL, KA ;
COCKERELL, CJ ;
PETRI, WA .
NEW ENGLAND JOURNAL OF MEDICINE, 1994, 330 (21) :1509-1515
[3]   CHARACTERIZATION OF A 17-KILODALTON ANTIGEN OF BARTONELLA-HENSELAE REACTIVE WITH SERA FROM PATIENTS WITH CAT-SCRATCH DISEASE [J].
ANDERSON, B ;
LU, E ;
JONES, D ;
REGNERY, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (09) :2358-2365
[4]   Comparative seroreactivity to Bartonella henselae and Bartonella quintana among cats from Israel and North Carolina [J].
Baneth, G ;
Kordick, DL ;
Hegarty, BC ;
Breitschwerdt, EB .
VETERINARY MICROBIOLOGY, 1996, 50 (1-2) :95-103
[5]   Predominance of two Bartonella henselae variants among cat-scratch disease patients in the Netherlands [J].
Bergmans, AMC ;
Schellekens, JFP ;
vanEmbden, JDA ;
Schouls, LM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (02) :254-260
[6]  
BERGMANS AMC, 1996, THESIS U UTRECHT UTR
[7]   ENDOCARDITIS IN A DOG DUE TO INFECTION WITH A NOVEL BARTONELLA SUBSPECIES [J].
BREITSCHWERDT, EB ;
KORDICK, DL ;
MALARKEY, DE ;
KEENE, B ;
HADFIELD, TL ;
WILSON, K .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) :154-160
[8]   PROPOSALS TO UNIFY THE GENERA BARTONELLA AND ROCHALIMAEA, WITH DESCRIPTIONS OF BARTONELLA-QUINTANA COMB-NOV, BARTONELLA-VINSONII COMB-NOV, BARTONELLA-HENSELAE COMB-NOV, AND BARTONELLA-ELIZABETHAE COMB-NOV, AND TO REMOVE THE FAMILY BARTONELLACEAE FROM THE ORDER RICKETTSIALES [J].
BRENNER, DJ ;
OCONNOR, SP ;
WINKLER, HH ;
STEIGERWALT, AG .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1993, 43 (04) :777-786
[9]  
CHILDS JE, 1994, J AM VET MED ASSOC, V204, P1775
[10]   PREVALENCE OF ANTIBODIES TO ROCHALIMAEA SPECIES (CAT-SCRATCH DISEASE AGENT) IN CATS [J].
CHILDS, JE ;
OLSON, JG ;
WOLF, A ;
COHEN, N ;
FAKILE, Y ;
ROONEY, JA ;
BACELLAR, F ;
REGNERY, RL .
VETERINARY RECORD, 1995, 136 (20) :519-520