Colorimetric detection of immunoglobulin G by use of functionalized gold nanoparticles on polyethylenimine film

被引：21

作者：

Li, HQ ^{[1
]}

Wang, CG ^{[1
]}

Ma, ZF ^{[1
]}

Su, ZM ^{[1
]}

机构：

[1] NE Normal Univ, Dept Chem, Changchun 130024, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2006年 / 384卷 / 7-8期

基金：

中国国家自然科学基金;

关键词：

immunoglobulin G; gold nanoparticles; polyethylenimine; immunosensor;

D O I：

10.1007/s00216-006-0309-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method based on use of functionalized gold nanoparticles on polyethylenimine film has been developed for colorimetric detection of immunoglobulin G (IgG). The immunogold nanoparticles were immobilized on quartz slides by recognition between antibody and antigen, with the antigen chemically adsorbed on the polyethylenimine film. By measurement of the UV-visible spectra of the immobilized immunogold, detection of h-IgG was achieved. The detection limit for h-IgG by use of this method can be as low as 0.01 mu g mL(-1). This method is quite promising for numerous applications in immunoassay.

引用

页码：1518 / 1524

页数：7

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