Chromosomal system for studying AmpC-mediated β-lactam resistance mutation in Escherichia coli

被引:18
作者
Petrosino, JF
Pendleton, AR
Weiner, JH
Rosenberg, SM
机构
[1] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA
[2] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
[3] Baylor Coll Med, Dept Mol Virol, Houston, TX 77030 USA
[4] Univ Alberta, Dept Biochem, Edmonton, AB T6G 2H7, Canada
关键词
D O I
10.1128/AAC.46.5.1535-1539.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In some enterobacterial pathogens, but not in Escherichia coli, loss-of-function mutations in the ampD gene are a common route to beta-lactam antibiotic resistance. We constructed an assay system for studying mechanism(s) of enterobacterial ampD mutation using the well-developed genetics of E. coli. We integrated the Enterobacter ampRC genes into the E. coli chromosome. These cells acquire spontaneous recombination- and SOS response-independent beta-lactam resistance mutations in ampD. This chromosomal system is useful for studying mutation mechanisms that promote antibiotic resistance.
引用
收藏
页码:1535 / 1539
页数:5
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