Identification of non-functional human VNO receptor genes provides evidence for vestigiality of the human VNO

被引:36
作者
Kouros-Mehr, H
Pintchovski, S
Melnyk, J
Chen, YJ
Friedman, C
Trask, B
Shizuya, H [1 ]
机构
[1] CALTECH, Beckman Inst, Div Biol, Pasadena, CA 91125 USA
[2] Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA
关键词
D O I
10.1093/chemse/26.9.1167
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
In mammals, the vomeronasal organ (VNO) contains chemosensory receptor cells that bind to pheromones and induce a variety of social and reproductive behaviors, It has been traditionally assumed that the human VNO (Jacobson's organ) is a vestigial structure, although recent studies have shown minor evidence for a structurally intact and possibly functional VNO, The presence and function of the human VNO remains controversial, however, as pheromones and VNO receptors have not been well characterized, In this study we screened a human Bacterial Artificial Chromosome (BAC) library with multiple primer sets designed from human cDNA sequences homologous to mouse VNO receptor genes. Utilizing these BAC sequences in addition to mouse VNO receptor sequences, we screened the High Throughput Genome Sequence (HTGS) database to find additional human putative VNO receptor genes. We report the identification of 56 BACs carrying 34 distinct putative VNO receptor gene sequences, all of which appear to be pseudogenes. Sequence analysis indicates substantial homology to mouse V1R and V2R VNO receptor families, Furthermore, chromosomal localization via FISH analysis and RH mapping reveal that the majority of the BACs are localized to telomeric and centromeric chromosomal localizations and may have arisen through duplication events. These data yield insight into the present state of pheromonal olfaction in humans and into the evolutionary history of human VNO receptors. (Sequence data from this article have been deposited with the DDBJ/EMBL/Genbank Data Libraries under accession nos. AF305393-305416.).
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页码:1167 / 1174
页数:8
相关论文
共 24 条
[1]   SENSORY RECEPTOR-LIKE CELLS IN THE HUMAN FETAL VOMERONASAL ORGAN [J].
BOEHM, N ;
GASSER, B .
NEUROREPORT, 1993, 4 (07) :867-870
[2]   The molecular architecture of odor and pheromone sensing in mammals [J].
Buck, LB .
CELL, 2000, 100 (06) :611-618
[3]   RADIATION HYBRID MAPPING - A SOMATIC-CELL GENETIC METHOD FOR CONSTRUCTING HIGH-RESOLUTION MAPS OF MAMMALIAN CHROMOSOMES [J].
COX, DR ;
BURMEISTER, M ;
PRICE, ER ;
KIM, S ;
MYERS, RM .
SCIENCE, 1990, 250 (4978) :245-250
[4]  
Doving KB, 1998, J EXP BIOL, V201, P2913
[5]   Activation of an anatomically distinct subpopulation of accessory olfactory bulb neurons by chemosensory stimulation [J].
Dudley, CA ;
Moss, RL .
NEUROSCIENCE, 1999, 91 (04) :1549-1556
[6]   Expression of candidate pheromone receptor genes in vomeronasal neurons [J].
Dulac, C ;
Axel, R .
CHEMICAL SENSES, 1998, 23 (04) :467-475
[7]   A NOVEL FAMILY OF GENES ENCODING PUTATIVE PHEROMONE RECEPTORS IN MAMMALS [J].
DULAC, C ;
AXEL, R .
CELL, 1995, 83 (02) :195-206
[8]   Characterization of nonfunctional V1R-like pheromone receptor sequences in human [J].
Giorgi, D ;
Friedman, C ;
Trask, BJ ;
Rouquier, S .
GENOME RESEARCH, 2000, 10 (12) :1979-1985
[9]   THE ORGANIZATION AND FUNCTION OF THE VOMERONASAL SYSTEM [J].
HALPERN, M .
ANNUAL REVIEW OF NEUROSCIENCE, 1987, 10 :325-362
[10]   A novel family of putative pheromone receptors in mammals with a topographically organized and sexually dimorphic distribution [J].
Herrada, G ;
Dulac, C .
CELL, 1997, 90 (04) :763-773