Preparation of succinimidyl and pentafluorophenyl active esters of 5- and 6-carboxyfluorescein

被引：33

作者：

Adamczyk, M

Fishpaugh, JR

Heuser, KJ

机构：

[1] Diagnostic Division, Division Organic Chemistry (D-9NM), Abbott Laboratories, Abbott Park, IL 60064-3500

来源：

BIOCONJUGATE CHEMISTRY | 1997年 / 8卷 / 02期

关键词：

D O I：

10.1021/bc9600877

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A mixture of 5- and 6-carboxyfluorescein was activated with 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride in the presence of either N-hydroxysuccinimide or pentafluorophenol to give the corresponding succinimidyl and pentafluorophenyl esters. The regioisomeric mixtures were separated to give the 5- and 6-succinimidyl and pentafluorophenyl active esters in >98% purity.

引用

页码：253 / 255

页数：3

共 13 条

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