Formation of a giant toroid from long duplex DNA

被引：53

作者：

Yoshikawa, Y

Yoshikawa, K ^{[1
]}

Kanbe, T

机构：

[1] Kyoto Univ, Grad Sch Sci, Dept Phys, Kyoto 6068502, Japan

[2] Nagoya Univ, Sch Med, Dis Mechanism & Control Res Inst, Med Mycol Lab, Nagoya, Aichi 4660064, Japan

[3] Nagoya Bunri Coll, Dept Food & Nutr, Nagoya, Aichi 4510077, Japan

来源：

LANGMUIR | 1999年 / 15卷 / 12期

关键词：

D O I：

10.1021/la981159g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

It is well-known that polyamines, such as spermidine, have the effect of condensing DNA molecules into a toroidal morphology with an outer diameter of 80-100 nm. We have investigated the collapse of long duplex T4 DNA induced by spermidine with use of fluorescence and electron microscopy. We found that giant toroids with an outer diameter of similar to 200 nm are generated from single T4 DNA under a high concentration of spermidine in the buffer solutions with rather high salt.

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页码：4085 / 4088

页数：4

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