The role of oxidative DNA damage in human arsenic carcinogenesis:: Detection of 8-hydroxy-2′-deoxyguanosine in arsenic-related Bowen's disease

被引:156
作者
Matsui, M
Nishigori, C [1 ]
Toyokuni, S
Takada, J
Akaboshi, M
Ishikawa, M
Imamura, S
Miyachi, Y
机构
[1] Kyoto Univ, Grad Sch Med, Dept Dermatol, Kyoto 6068507, Japan
[2] Kyoto Univ, Grad Sch Med, Dept Pathol & Biol Dis, Kyoto 6068507, Japan
[3] Kyoto Univ, Inst Res Reactor, Osaka 59004, Japan
[4] Yaizu City Hosp, Shizuoka, Japan
关键词
neutron activation analysis;
D O I
10.1046/j.1523-1747.1999.00630.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Arsenic is widely distributed in nature in the form of either metalloids or chemical compounds, which cause a variety of pathologic conditions including cutaneous and visceral malignancies. Recently, reactive oxygen species have been hypothesized to be one of the causes of arsenic-induced carcinogenesis. 8-Hydroxy-2'-deoxyguanosine is one of the major reactive oxygen species-induced DNA base-modified products that is widely accepted as a sensitive marker of oxidative DNA damage. We studied the presence of 8-hydroxy2'-deoxyguanosine by immunohistochemistry using N45.1 monoclonal antibody in 28 cases of arsenic-related skin neoplasms and arsenic keratosis as well as in 11 cases of arsenic-unrelated Bowen's diseases. The frequency of 8-hydroxy-2'-deoxyguanosine positive cases was significantly higher in arsenic-related skin neoplasms (22 of 28; 78%) than in arsenic-unrelated Bowen's disease (one of 11; 9%) (p < 0.001 by chi(2) test). 8-Hydroxy-2'-deoxyguanosine was also detected in normal tissue adjacent to the arsenic-related Bowen's disease lesions. Furthermore, arsenic was detected by neutron activation analysis in the deparaffined skin tumor samples of arsenic-related disease (four of five; 80%), whereas arsenic was not detected in control samples. Our results strongly suggest the involvement of reactive oxygen species in arsenic-induced human skin cancer.
引用
收藏
页码:26 / 31
页数:6
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