Structural analysis of non-native states of proteins by NMR methods

被引：182

作者：

Shortle, DR

机构：

[1] Department of Biological Chemistry, Johns Hopkins Univ. Sch. of Medicine, Baltimore, MD 21205

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1996年 / 6卷 / 01期

关键词：

D O I：

10.1016/S0959-440X(96)80091-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Established NMR methods are increasingly being applied to the non-native states of proteins. For small denatured proteins, full assignment of proton, N-15 and C-13 resonances is often straightforward. Sensitive methods exist for detecting fractionally populated alpha helices and beta strands, but defining transient interactions among side chains is proving more problematic. The non-native states of several small proteins are being intensively investigated to address a number of questions about protein folding.

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页码：24 / 30

页数：7

相关论文

共 58 条

[1] NMR STRUCTURE OF A STABLE OB-FOLD SUBDOMAIN ISOLATED FROM STAPHYLOCOCCAL NUCLEASE [J].

ALEXANDRESCU, AT ;

GITTIS, AG ;

ABEYGUNAWARDANA, C ;

SHORTLE, D .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 250 (02) :134-143

[2] BACKBONE DYNAMICS OF A HIGHLY DISORDERED 131-RESIDUE FRAGMENT OF STAPHYLOCOCCAL NUCLEASE [J].

ALEXANDRESCU, AT ;

SHORTLE, D .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 242 (04) :527-546

[3] STRUCTURE AND DYNAMICS OF A DENATURED 131-RESIDUE FRAGMENT OF STAPHYLOCOCCAL NUCLEASE - A HETERONUCLEAR NMR-STUDY [J].

ALEXANDRESCU, AT ;

ABEYGUNAWARDANA, C ;

SHORTLE, D .

BIOCHEMISTRY, 1994, 33 (05) :1063-1072

[4] CHARACTERIZATION OF A TRIFLUOROETHANOL-INDUCED PARTIALLY FOLDED STATE OF ALPHA-LACTALBUMIN [J].

ALEXANDRESCU, AT ;

NG, YL ;

DOBSON, CM .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 235 (02) :587-599

[5] ASSOCIATION OF BIOMOLECULAR SYSTEMS VIA PULSED-FIELD GRADIENT NMR SELF-DIFFUSION MEASUREMENTS [J].

ALTIERI, AS ;

HINTON, DP ;

BYRD, RA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (28) :7566-7567

[6] TOWARD SOLVING THE FOLDING PATHWAY OF BARNASE - THE COMPLETE BACKBONE C-13, N-15, AND H-1-NMR ASSIGNMENTS OF ITS PH-DENATURED STATE [J].

ARCUS, VL ;

VUILLEUMIER, S ;

FREUND, SMV ;

BYCROFT, M ;

FERSHT, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (20) :9412-9416

[7]

ARCUS VL, 1996, IN PRESS J MOL BIOL

[8] PRIMARY STRUCTURE EFFECTS ON PEPTIDE GROUP HYDROGEN-EXCHANGE [J].

BAI, YW ;

MILNE, JS ;

MAYNE, L ;

ENGLANDER, SW .

PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1993, 17 (01) :75-86

[9] PROTEIN-FOLDING INTERMEDIATES - NATIVE-STATE HYDROGEN-EXCHANGE [J].

BAI, YW ;

SOSNICK, TR ;

MAYNE, L ;

ENGLANDER, SW .

SCIENCE, 1995, 269 (5221) :192-197

[10]

BALBACH J, 1996, IN PRESS NATURE STRU

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