Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis

被引:57
作者
Haijema, BJ
vanSinderen, D
Winterling, K
Kooistra, J
Venema, G
Hamoen, LW
机构
[1] UNIV GRONINGEN,DEPT GENET,GRONINGEN BIOMOL SCI & BIOTECHNOL INST,NL-9751 NN HAREN,NETHERLANDS
[2] UNIV MARYLAND BALTIMORE CTY,BALTIMORE,MD 21228
关键词
D O I
10.1111/j.1365-2958.1996.tb02657.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It has been hypothesized that the dinR gene product of Bacillus subtilis acts as a repressor of the SOS regulon by binding to DNA sequences located upstream of SOS genes, including dinR and recA. Following activation as a result of DNA damage, RecA is believed to catalyse DinR-autocleavage, thus derepressing the SOS regulon. The present results support this hypothesis: a dinR insertion mutation caused a high, constitutive expression of both dinR and recA, which could not be further elevated by SOS-induction. In addition, gel-retardation assays demonstrated a direct interaction between the dinR gene product and the recA and dinR promoter regions. Epistatic interactions and gel-retardation assays demonstrated that the previously reported competence-specific expression of recA directly depended upon the gene product of comK, the competence transcription factor. These data demonstrate the existence of a direct regulatory link between the competence signal-transduction pathway and the SOS regulon.
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收藏
页码:75 / 85
页数:11
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