Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages

被引：71

作者：

Hashimoto, Mayu

Morimoto, Minoru

Saimoto, Hiroyuki

Shigemasa, Yoshihiro

Yanagie, Hironobu

Eriguchi, Masazumi

Sato, Toshinori

机构：

[1] Keio Univ, Dept Biosci & Informat, Yokohama, Kanagawa 2238522, Japan

[2] Tottori Univ, Dept Mat Sci, Tottori 6808552, Japan

[3] Univ Tokyo, Adv Sci & Technol Res Ctr, Tokyo 1538904, Japan

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 11期

关键词：

mannose; chitosan; gene transfer; receptor-mediated endocytosis; macrophage; plasmid DNA;

D O I：

10.1007/s10529-006-9006-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.

引用

页码：815 / 821

页数：7

共 22 条

[1] Mechanisms of phagocytosis in macrophages [J].