Genotypic differentiation of Gardnerella vaginalis by amplified ribosomal DNA restriction analysis (ARDRA)

被引：35

作者：

Ingianni, A ^{[1
]}

Petruzzelli, S ^{[1
]}

Morandotti, G ^{[1
]}

Pompei, R ^{[1
]}

机构：

[1] CATHOLIC UNIV ROME,IST MICROBIOL,I-00168 ROME,ITALY

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 1997年 / 18卷 / 01期

关键词：

Gardnerella vaginalis; PCR-ribotyping; amplified ribosomal DNA restriction analysis (ARDRA); MOLECULAR EPIDEMIOLOGY; RNA SEQUENCES; IDENTIFICATION; COMPILATION; BACTERIA;

D O I：

10.1111/j.1574-695X.1997.tb01028.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In total 34 strains of Gardnerella vaginalis were analyzed with various molecular techniques in order to find the possibility of dividing this single species into different genotypes. Classical ribotyping, PCR-ribotyping and restriction analysis of 16S-23S rRNA intergenic spacer sequences were all unsuccessful in genotype differentiation of these bacteria. Only amplified ribosomal DNA restriction analysis (ARDRA) was suitable in recognizing different G. vaginalis genotypes. At least 3-4 genotypes were identified with different restriction enzymes, some of which showed a prevalent distribution in certain of the centers from which they were collected. Although in this study no correlation was found between bacterial vaginosis and any of the genotypes identified, the ARDRA method could prove to be a useful tool for studying the etiopathology and epidemiology of G. vaginalis.

引用

页码：61 / 66

页数：6

共 18 条

[1] GARDNERELLA-VAGINALIS - CHARACTERISTICS, CLINICAL CONSIDERATIONS, AND CONTROVERSIES [J].

CATLIN, BW .

CLINICAL MICROBIOLOGY REVIEWS, 1992, 5 (03) :213-237

[2]

Clarridge Jill E., 1995, P357

[3] RIBOSOMAL RIBONUCLEIC-ACID GENE RESTRICTION PATTERNS AS POTENTIAL TAXONOMIC TOOLS [J].

GRIMONT, F ;

GRIMONT, PAD .

ANNALES DE L INSTITUT PASTEUR-MICROBIOLOGIE, 1986, 137B (02) :165-175

[4] A COMPILATION OF LARGE SUBUNIT (23S-LIKE) RIBOSOMAL-RNA SEQUENCES PRESENTED IN A SECONDARY STRUCTURE FORMAT [J].

GUTELL, RR ;

SCHNARE, MN ;

GRAY, MW .

NUCLEIC ACIDS RESEARCH, 1990, 18 :2319-2330

[5] RAPID IDENTIFICATION OF BACTERIA ON THE BASIS OF POLYMERASE CHAIN REACTION-AMPLIFIED RIBOSOMAL DNA SPACER POLYMORPHISMS [J].

JENSEN, MA ;

WEBSTER, JA ;

STRAUS, N .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (04) :945-952

[6] A UNIVERSAL APPROACH TO BACTERIAL MOLECULAR EPIDEMIOLOGY BY POLYMERASE CHAIN-REACTION RIBOTYPING [J].

KOSTMAN, JR ;

ALDEN, MB ;

MAIR, M ;

EDLIND, TD ;

LIPUMA, JJ ;

STULL, TL .

JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (01) :204-208

[7] MOLECULAR EPIDEMIOLOGY OF PSEUDOMONAS-CEPACIA DETERMINED BY POLYMERASE CHAIN-REACTION RIBOTYPING [J].

KOSTMAN, JR ;

EDLIND, TD ;

LIPUMA, JJ ;

STULL, TL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (08) :2084-2087

[8] MOLECULAR EPIDEMIOLOGY - APPLICATION OF CONTEMPORARY TECHNIQUES TO THE TYPING OF MICROORGANISMS [J].

MASLOW, JN ;

MULLIGAN, ME ;

ARBEIT, RD .

CLINICAL INFECTIOUS DISEASES, 1993, 17 (02) :153-164

[9] THE CHARACTERIZATION OF GARDNERELLA-VAGINALIS DNA USING NONRADIOACTIVE DNA PROBES [J].

NATH, K ;

CHOI, DJ ;

DEVLIN, D .

RESEARCH IN MICROBIOLOGY, 1991, 142 (05) :573-583

[10] HETEROGENEITY IN RESTRICTION PATTERNS OF GARDNERELLA-VAGINALIS ISOLATED FROM INDIVIDUALS WITH BACTERIAL VAGINOSIS [J].

NATH, K ;

DEVLIN, D ;

BEDDOE, AM .

RESEARCH IN MICROBIOLOGY, 1992, 143 (02) :199-209

← 1 2 →