Terpenoids from Eupatorium fortunei TURCZ

Four new terpenoids, namely, rel-(1R,2S,3R,4R,6S)-p-menthane-1,2,3,6-tetrol (1), rel(1R,2R,3R,4S,6S)-p-menthane-1,2,3,6-tetrol (2), 9-hydroxythymol 3-O-angelate (3), and (3 beta, 20R)-20-hydroxylanost-25-en-3-yl palmitate (4), together with fourteen known compounds, were isolated from the AcOEt part of the MeOH extracts of Eupatorium fortunei. In addition, two other monoterpenoids, 'acetone thymol-8,9-diyl ketal' (19) and 8-methoxy-9-hydroxythymol 3-O-angelate (20) were also obtained which were probably artifacts but have never been reported in the literature. The structures of the new compounds, including their relative configurations, were established by an extensive study of their spectral data, especially 1D- and 2D-NMR. The cytotoxic activity of the new compounds against human hepatoma (SMMC-7721), human leukemia (HL-60), and human hepatocyte (LO2) cells was investigated.