Distribution of Methanotrophs in the Phyllosphere

被引:32
作者
Iguchi, Hiroyuki [1 ]
Sato, Izuru [1 ]
Sakakibara, Maiko [1 ]
Yurimoto, Hiroya [1 ]
Sakai, Yasuyoshi [1 ]
机构
[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan
基金
日本学术振兴会;
关键词
methanotroph; methylotroph; methane; methanol; plant; PARTICULATE METHANE MONOOXYGENASE; BACTERIA; POPULATIONS; EMISSIONS; FLUXES; PLANTS; SOILS;
D O I
10.1271/bbb.120281
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plants have been reported to emit methane as well as methanol originating in their cell-wall constituents. We investigated methanotrophs in the phyllosphere by the enrichment culture method with methane as sole carbon source. We enriched methanotrophs from the leaves, flowers, bark, and roots of various plants. Analysis of the pmoA and mxaF genes retrieved from the enrichment cultures revealed that methanotrophs closely related to the genera Methylomonas, Methylosinus, and Methylocystis inhabit not only the rhizosphere but also the phyllosphere, together with methanol-utilizing bacteria.
引用
收藏
页码:1580 / 1583
页数:4
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