Production of bicyclic and tricyclic triterpenes by mutated squalene-hopene cyclase

被引：37

作者：

Pale-Grosdemange, C

Merkofer, T

Rohmer, M

Poralla, K

机构：

[1] Univ Tubingen, D-72076 Tubingen, Germany

[2] Univ Strasbourg, Inst Le Bel, CNRS, F-67070 Strasbourg, France

来源：

TETRAHEDRON LETTERS | 1999年 / 40卷 / 33期

关键词：

hopene; malabaricatriene; polypodatetraene; squalene cyclization; triterpene synthase;

D O I：

10.1016/S0040-4039(99)01248-4

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Mutagenesis of Tyr420 to Ala in the catalytic cavity of squalene-hopene cyclase of Alicyclobacillus acidocaldarius resulted in an altered product spectrum. Besides hopene and diplopterol, this mutant produced significant amounts of the bicyclic alpha- and gamma-polypodatetraenes and minor amounts of the tricyclic 13 alpha(H)-malabaricatriene. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：6009 / 6012

页数：4

共 14 条

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