Vertical structure of archaeal communities and the distribution of ammonia monooxygenase A gene variants in two meromictic High Arctic lakes

被引:91
作者
Pouliot, Jeremie [3 ]
Galand, Pierre E. [3 ,4 ]
Lovejoy, Connie [3 ]
Vincent, Warwick F. [1 ,2 ]
机构
[1] Univ Laval, Dept Biol, Quebec City, PQ G1V 0A6, Canada
[2] Univ Laval, Ctr Etud Nord, Quebec City, PQ G1V 0A6, Canada
[3] Univ Laval, Dept Biol & Quebec Ocean, Quebec City, PQ G1V 0A6, Canada
[4] CSIC, Limnol Unit, Dept Continental Ecol, Ctr Estudis Avancats Blanes, Blanes 17300, Spain
基金
加拿大自然科学与工程研究理事会;
关键词
MCMURDO DRY VALLEYS; BLACK-SEA; MOLECULAR ANALYSIS; ELLESMERE ISLAND; NITROUS-OXIDE; SODA LAKE; MONO LAKE; DIVERSITY; OCEAN; CRENARCHAEOTA;
D O I
10.1111/j.1462-2920.2008.01846.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The distribution of archaeal amoA and 16S rRNA genes was evaluated in two marine-derived, meromictic lakes in the Canadian High Arctic: Lake A and Lake C1 on the northern coast of Ellesmere Island. The amoA gene was recorded in both lakes, with highest copy numbers in the oxycline. Sequence analysis showed that amoA from the two lakes shared 94% similarity, indicating at least two phylogenetically distinct clusters. Clone libraries of archaeal 16S rRNA genes from Lake A revealed strong vertical differences in archaeal community diversity and composition down the water column. The oxic layer was dominated by one group of Euryarchaeota affiliated to the Lake Dagow Sediment (LDS) cluster. This group was absent from the oxycline, which had an extremely low archaeal diversity of two phylotypes. Both belonged to the Crenarchaeota Marine Group I (MGI), the marine group that has been linked to archaeal amoA; however, there was a low ratio of amoA to MGI copy numbers, suggesting that many MGI Archaea did not carry the amoA gene. The anoxic zone contained representatives of the RC-V (Rice Cluster-V) and LDS clusters of Euryarchaeota. These results show the strong vertical differentiation of archaeal communities in polar meromictic lakes, and they suggest archaeal nitrification within the oxycline of these highly stratified waters.
引用
收藏
页码:687 / 699
页数:13
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